| 中国柯萨奇病毒A组6型分离株全基因序列特征分析 |
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| 引用本文： | 董小聪,纪望全,陈帅印,杨海燕,段广才,晋乐飞.中国柯萨奇病毒A组6型分离株全基因序列特征分析[J].中华疾病控制杂志,2022,26(6):740-744. |
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| 作者姓名： | 董小聪 纪望全 陈帅印 杨海燕 段广才 晋乐飞 |
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| 作者单位： | 450001 郑州，郑州大学公共卫生学院流行病学教研室 |
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| 基金项目： | 国家自然科学基金项目82002147国家自然科学基金项目82073618中国博士后科学基金项目2019M662543河南省高等学校重点科研项目20A330004河南省高等学校重点科研项目21A310026 |
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| 摘 要： | 目的 分析中国2007-2019年柯萨奇病毒A组6型(coxsackievirus A6，CVA6)毒株遗传进化规律，以期为疾病预防及疫苗研发提供理论依据。 方法 收集中国截至2021年3月1日Genbank数据库中454株CVA6毒株全基因序列，分析毒株分离年份、地区信息，构建系统发育树揭示进化规律并进行基因重组分析。 结果 地区信息显示，毒株主要分布于中国沿海及南方地区，时间信息表明，自2013年起，CVA6毒株报告数量逐渐增加。系统进化结果表明，454株CVA6毒株分10个分支，A分支中3株分离自江西省的毒株与CVA6原型毒株Gdula(AY421764)亲缘关系极为接近，核酸序列相似度近乎100%，J分支是中国流行最广泛的毒株群，包含了中国较多省份如山东省、广东省等。基因重组分析显示，基因重组现象较为普遍，大部分CVA6代表毒株在P2区和P3区与其他肠道病毒A组毒株之间存在基因重组现象。 结论 2007-2019年CVA6毒株在中国广泛传播，毒株间基因重组现象普遍存在。
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| 关 键 词： | 手足口病 CVA6 系统进化 基因重组 |
| 收稿时间： | 2021-07-29 |
The complete gene sequence characteristic analysis of coxsackievirus A6 strains isolated from China |
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| Affiliation: | Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, China |
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| Abstract: | Objective To analyze the genetic evolutionary characteristic of coxsackievirus A6 (CVA6) strains reported from 2007 to 2019 of China to provide theoretical basis for disease prevention and development of vaccines. Methods The whole information of gene sequences of 454 Chiness CVA6 strains reported up to March 1, 2021 were collected from Genbank, and the isolation year and region of the strains were analyzed. Phylogenetic tree was constructed to reveal the evolutionary character of the strains and gene recombination analysis was conducted. Results The information of isolated region showed that the CVA6 strains were mainly originated from coastal areas and south of China. The number of reported CVA6 strains had a significant increase since 2013. The phylogenetic tree revealed that 454 CVA6 strains were mainly clustered into 10 branches. Three strains in Group A isolated from Jiangxi Province was most closely related to the CVA6 prototype strain Gdula (AY421764), with nearly 100% similarity of nucleic acid sequence. Group J isolated from many provinces in China, such as Shandong Province and Guangdong Province, was the most widespread group of strains. Recombination analysis showed that gene recombination of most strains was common. Most representative strains of CVA6 were recombined with some other Enterovirus A strains in P2 and P3 regions. Conclusion From 2007 to 2019, the CVA6 strains have been spreading widely in China, and the phenomenon of gene recombination among CVA6 strains is widespread. |
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