| 姜黄素联合KLF8-siRNA干扰对肺癌A549细胞增殖、迁移和凋亡的影响及其机制 |
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| 引用本文： | 谷见法,刘松格,潘琼,闫巧辉. 姜黄素联合KLF8-siRNA干扰对肺癌A549细胞增殖、迁移和凋亡的影响及其机制[J]. 现代肿瘤医学, 2022, 0(4): 589-594. DOI: 10.3969/j.issn.1672-4992.2022.04.006 |
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| 作者姓名： | 谷见法 刘松格 潘琼 闫巧辉 |
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| 作者单位： | 郑州大学附属郑州中心医院肿瘤内科,河南 郑州 450000 |
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| 摘 要： | 目的:探讨姜黄素联合KLF8-siRNA干扰对肺癌A549细胞增殖、迁移和凋亡的影响及其机制.方法:采用脂质体法将KLF8-siRNA干扰序列转染至体外培养的A549细胞中,RT-PCR和Western blot检测转染效果.实验分为NC组(转染阴性对照)、KLF8-siRNA组(转染KLF8-siRNA)、Cur组(...
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| 关 键 词： | 肺癌 KLF8 姜黄素 增殖 迁移 凋亡 Wnt/β-catenin信号通路 |
Effects of curcumin combined wih KLF8-siRNA interference on proliferation,migration and apoptosis of lung cancer A549 cells and its mechanism |
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| GU Jianfa,LIU Songge,PAN Qiong,YAN Qiaohui. Effects of curcumin combined wih KLF8-siRNA interference on proliferation,migration and apoptosis of lung cancer A549 cells and its mechanism[J]. Journal of Modern Oncology, 2022, 0(4): 589-594. DOI: 10.3969/j.issn.1672-4992.2022.04.006 |
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| Authors: | GU Jianfa LIU Songge PAN Qiong YAN Qiaohui |
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| Affiliation: | Department of Oncology,Zhengzhou Central Hospital,Zhengzhou University,Henan Zhengzhou 450000,China. |
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| Abstract: | Objective:To investigate the effects of curcumin combined with KLF8-siRNA interference on proliferation,migration and apoptosis of lung cancer A549 cells and its mechanism.Methods:KLF8-siRNA interference sequence was transfected into A549 cells by liposome method,and the transfection effects were detected by RT-PCR and Western blot.The experiment was divided into NC group(negative control),KLF8-siRNA group(transfected KLF8-siRNA),Cur group(treated with 40μmol/L curcumin)and KLF8-siRNA+Cur group(treated with curcumin after transfected KLF8-siRNA).The cell proliferation was detected by MTT and plate cloning.Cell migration was measured by Transwell chamber and scratch test.Cell apoptosis was detected by flow cytometry,and the expression of Wnt/β-catenin signaling pathway related proteinsβ-catenin,c-Myc,CyclinD1,MMP-2 and Survivin was detected by Western blot.Results:After transfection of KLF8-siRNA,the expression of KLF8 mRNA and protein in A549 cells decreased significantly.Compared with NC group,KLF8-siRNA transfection or curcumin treatment significantly reduced cell survival,clone formation,migration cell number and migration rate,while cell apoptosis rate significantly increased.Meanwhile,the expression levels ofβ-catenin,c-Myc,CyclinD1,MMP-2 and Survivin protein were significantly decreased(P<0.05).The above effects of curcumin combined with KLF8-siRNA interference on A549 cells were more significant than that of curcumin or KLF8-siRNA interference alone(P<0.05).Conclusion:Both KLF8-siRNA interference and curcumin can inhibit the proliferation,migration and promote apoptosis of A549 cells,and their combined effect is stronger.The mechanism may be related to the co-inhibition of Wnt/β-catenin signaling pathway activation. |
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| Keywords: | lung cancer KLF8 curcumin proliferation migration apoptosis Wnt/β-catenin signaling pathway |
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