| ALMS 1-IT1在结直肠癌中的表达及其对癌细胞增殖、侵袭和上皮间质转化的影响 |
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| 引用本文： | 王浩艺,刘畅,孙学军. ALMS 1-IT1在结直肠癌中的表达及其对癌细胞增殖、侵袭和上皮间质转化的影响[J]. 现代肿瘤医学, 2022, 0(4): 561-566. DOI: 10.3969/j.issn.1672-4992.2022.04.001 |
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| 作者姓名： | 王浩艺 刘畅 孙学军 |
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| 作者单位： | 1.西安交通大学第一附属医院普通外科，陕西 西安 710061；2.榆林市第一医院普通外科，陕西 榆林 718099 |
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| 基金项目： | 国家自然科学基金资助项目(编号:81972720)。 |
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| 摘 要： | 目的:探讨lncRNA ALMS1-IT1在结直肠癌(colorectal cancer,CRC)中的表达及预后意义,明确ALMS1-IT1对CRC细胞增殖、侵袭和上皮间质转化(epithelial-mesenchymal transition,EMT)的影响.方法:通过GEPIA网络平台分析癌症基因图谱(The Ca...
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| 关 键 词： | ALMS1-IT1 结直肠癌 增殖 迁移 侵袭 上皮间质转化 |
The expression of ALMS1-IT1 in colorectal cancer and its effect on cancer cell proliferation,invasion and epithelial-mesenchymal transition |
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| WANG Haoyi,LIU Chang,SUN Xuejun. The expression of ALMS1-IT1 in colorectal cancer and its effect on cancer cell proliferation,invasion and epithelial-mesenchymal transition[J]. Journal of Modern Oncology, 2022, 0(4): 561-566. DOI: 10.3969/j.issn.1672-4992.2022.04.001 |
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| Authors: | WANG Haoyi LIU Chang SUN Xuejun |
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| Affiliation: | 1.Department of General Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China;2.Department of General Surgery,the First Hospital of Yulin,Shaanxi Yulin 718099,China. |
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| Abstract: | Objective:To explore the expression and prognostic significance of lncRNA ALMS1-IT1 in colorectal cancer(CRC)and determine the impact of ALMS1-IT1 in CRC cell proliferation,invasion and epithelial-mesenchymal transition(EMT).Methods:The expression of ALMS1-IT1 in CRC and its correlation with the survival of patients were analyzed by GEPIA web server using The Cancer Genome Atlas(TCGA)database.The expression of ALMS1-IT was detected by RT-qPCR in human normal intestinal epithelial cells(NCM460)and CRC cells(HCT116,HT29,LoVo,SW480).ALMS1-IT was silenced and overexpressed by plasmid transfection in LoVo and SW480 cells,respectively.CCK-8 assay detected cell proliferation ability.Transwell assay analyzed cell migration and invasion potentials.Immunoblotting was used to detect the changes of EMT markers(E-cadherin,N-cadherin,Vimentin).Results:TCGA data analysis confirmed that ALMS1-IT expression in CRC tissues was significantly higher than that in normal tissues,and high expression of ALMS1-IT1 was prominently associated with poor prognosis.The expression of ALMS1-IT1 in CRC cells(HCT116,HT29,LoVo,SW480)was markedly higher than that in normal intestinal epithelial cells(NCM460).ALMS1-IT1 silencing inhibited cell proliferation,migration,invasion,and led to increased expression of E-cadherin protein and decreased expression of N-cadherin and Vimentin protein in LoVo cells.Overexpression of ALMS1-IT1 promoted cell proliferation,migration,invasion,and resulted in decreased expression of E-cadherin protein and increased expression of N-cadherin and Vimentin protein in SW480 cells.Conclusion:ALMS1-IT1 is highly expressed in CRC tissues and is closely related to poor prognosis.ALMS1-IT1 exerts an oncogenic role by promoting the proliferation,invasion and EMT of CRC cells. |
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| Keywords: | ALMS1-IT1 colorectal cancer proliferation migration invasion EMT |
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