| 约氏疟原虫与伯氏疟原虫侵入期抗原的初步研究 |
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| 引用本文： | 金立群,骆建民,傅玉才,许世锷,郭衍,谢霖崇,坪井敬文,鸟居本美.约氏疟原虫与伯氏疟原虫侵入期抗原的初步研究[J].中国寄生虫学与寄生虫病杂志,2003,21(5):272-274. |
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| 作者姓名： | 金立群 骆建民 傅玉才 许世锷 郭衍 谢霖崇 坪井敬文 鸟居本美 |
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| 作者单位： | 1. 汕头大学医学院寄生虫学教研室,汕头,515031
2. 日本爱媛大学医学部寄生虫学教研室 |
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| 基金项目： | 广东省卫生厅和李嘉诚基金会资助项目 (A1 99840 0 )~~ |
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| 摘 要： | 目的 用针对鼠约氏疟原虫(Plasmodiumyoelii)侵入期的8种单克隆抗体,对约氏疟原虫和伯氏疟原虫(P.berghei)侵入期即动合子、裂殖子和子孢子棒状体和表面抗原检测分析。 方法 间接免疫荧光实验(IFA)对各侵入期抗原进行亚细胞结构定位,SDSPAGE及Western印迹对两种鼠疟原虫的不同侵入期进行抗原组分分析。 结果 经上述两种方法检测发现,顶端复合体抗原成分复杂,约氏疟原虫和伯氏疟原虫的棒状体有共同的抗原表位,约氏疟原虫的动合子与其自身的裂殖子有类似成分,也有各自独特的抗原。两种鼠疟原虫动合子抗原有类似成分。约氏疟原虫的子孢子具有与裂殖子、动合子不同的抗原成分。 结论 疟原虫侵入期棒状体和表面抗原在同一虫种的不同侵入期和不同虫种中有共同的抗原表位,也有各自的独特组分。
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| 关 键 词： | 疟原虫 抗原表位 侵入期 |
| 文章编号： | 1000-7423(2003)-05-0272-03 |
| 修稿时间： | 2003年3月31日 |
Studies on the Antigens of Invasive Stages of Plasmodium yoelii and Plasmodium berghei |
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| JIN Li-qun,LUO Jian-min,FU Yu-cai,XU Shi-e,GUO Yan,XIE Lin-chong,T. Tsuboi,M. Torii.Studies on the Antigens of Invasive Stages of Plasmodium yoelii and Plasmodium berghei[J].Chinese Journal of Parasitology and Parasitic Diseases,2003,21(5):272-274. |
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| Authors: | JIN Li-qun LUO Jian-min FU Yu-cai XU Shi-e GUO Yan XIE Lin-chong T Tsuboi M Torii |
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| Affiliation: | Department of Parasitology, Shantou University Medical College, Shantou 515031. |
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| Abstract: | Objective To detect the rhoptry and surface proteins of invasive stages of Plasmodium yoelii and P. berghei with monoclonal antibodies. Methods Subcellular localization of antigens was detected by IFA. The antigens of different stages of the two species malaria parasites were analyzed by Western blotting. Results The antigens of rhoptry are very complicated. There are similar epitopes of the rhoptry proteins detected between the two species of Plasmodium. The similar epitopes were also detected between ookinetes and merozoites of P. yoelii, and ookinete antigens between the two species. But there are different antigens detected between merozoites and ookinetes in P. yoelii. The sporozoite surface antigen of P. yoelii was not detected in the ookinetes and merozoites in the same species. Conclusion There are similar epitopes in the rhoptry and surface antigens of different stages and different species of rodent malaria parasites. There are also distinct antigens among them. |
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| Keywords: | Plasmodium yoelii P berghei epitope of antigen invasive stage |
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