| 柴胡皂苷a影响大鼠急性脊髓损伤后机体炎症水平表达的实验研究 |
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| 引用本文： | 朱双龙,,,陈旭义,段会全,,刘迎节,,孙中磊,徐云强,.柴胡皂苷a影响大鼠急性脊髓损伤后机体炎症水平表达的实验研究[J].天津医科大学学报,2017,0(4):300-303. |
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| 作者姓名： | 朱双龙 陈旭义 段会全 刘迎节 孙中磊 徐云强 |
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| 作者单位： | (1.天津医科大学研究生院，天津 300070；2.天津医科大学总医院骨科,天津300052；3.武警后勤学院附属医院脑科中心 ,天津 300162； 4.锦州医科大学研究生院,锦州121000） |
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| 摘 要： |
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| 关 键 词： | 急性脊髓损伤 柴胡皂苷a 肿瘤坏死因子α 白介素6 大鼠 |
Effect of saikosaponina on expression of inflammation in rats after acute spinal cord injury |
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| ZHU Shuang-long,,' target="_blank" rel="external">,CHEN Xu-yi,DUAN Hui-quan,' target="_blank" rel="external">,LIU Ying-jie,' target="_blank" rel="external">,SUN Zhong-lei,XU Yun-qiang,' target="_blank" rel="external">.Effect of saikosaponina on expression of inflammation in rats after acute spinal cord injury[J].Journal of Tianjin Medical University,2017,0(4):300-303. |
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| Authors: | ZHU Shuang-long " target="_blank">' target="_blank" rel="external"> CHEN Xu-yi DUAN Hui-quan " target="_blank">' target="_blank" rel="external"> LIU Ying-jie " target="_blank">' target="_blank" rel="external"> SUN Zhong-lei XU Yun-qiang " target="_blank">' target="_blank" rel="external"> |
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| Abstract: | Objective: To investigate the effect of saikosaponina on the expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the early stage of acute spinal cord injury in rats. Methods: Thirty SD rats were randomly divided into sham operation group, simple injury group, saikosaponin a 5 mg / kg group, 10 mg / kg group, 20 mg / kg group, respectively. The acute spinal cord injury model was made by modified ，Allen，s heavy blow method. The rats in the injury group were injected with normal saline only, and the drug groups were intraperitoneally injected with the different dosages. All the animals were sacrificed at 24 h after the operation. The spinal cord and the vena cava sera of the injured segment were taken for 10 minutes and 2 minutes， respectively. The levels of TNF-αand IL-6 in the spinal cord tissue and serum were detected by enzyme-linked immunosorbent assay（ELISA）. Results: TNF-αand IL-6 in the spinal cord tissue and serum of the rats in the pure injury group were higher than those in the sham group (P <0.05). The levels of TNF-αand IL-6 in spinal cord tissue and serum of rats treated with saikosaponin a 5 mg / kg, 10 mg / kg and 20 mg / kg were lower than those of the injury group, and the difference was statistically significant (P < 0.05). The expressions of TNF-α and IL-6 in the spinal cord tissue of rats treated with 10 mg / group and 20 mg / kg group the difference were not statistically significant (P> 0.05). The levels of TNF-α and IL-6 in serum of rats treated with 10mg / kg and 20 mg/kg were lower than those of 5mg/kg group (P> 0.05). Conclusion: Saikosaponina could reduce the secondary immune inflammatory response by affecting the early expression of inflammatory factors to improve the body’s immune function in acute spinal cord injury. The10mg/kg group is superior to the other groups, and the drug may partially pass through the spinal blood barrier. |
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