| 阿奇霉素对哮喘致敏大鼠气道炎症及Th1/Th2失衡的调节作用 |
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| 引用本文： | 郭华,谭显曙,刘立涛,万莉红.阿奇霉素对哮喘致敏大鼠气道炎症及Th1/Th2失衡的调节作用[J].现代临床医学,2012,38(3):176-178. |
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| 作者姓名： | 郭华 谭显曙 刘立涛 万莉红 |
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| 作者单位： | 1. 成都市第六人民医院,四川成都,610051
2. 四川大学华西基础与法医学院,四川成都,610041 |
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| 摘 要： | 目的:探讨阿奇霉素对哮喘(OVA)致敏大鼠气道炎症及Th1/Th2失衡的调节作用。方法:SD大鼠40只,随机分为生理盐水组、哮喘模型组、地塞米松组以及阿奇霉素组,每组10只。利用卵白蛋白(Ovalbumin,OVA)/Al(OH)3致敏与OVA雾化吸入激发建立大鼠过敏性气道炎症模型,收集肺泡灌洗液(BALF)进行白细胞分类计数。采用ELISA法测定肺泡灌洗液中IL-2、IL-4、TNF-α与ET-1的表达情况。光镜观察肺组织病理结构变化。结果:OVA模型大鼠肺泡灌洗液中的中性粒细胞、淋巴细胞以及嗜酸性粒细胞含量明显增加;HE染色观察肺组织病理结构出现明显的支气管上皮脱落、杯状细胞增生,支气管周围嗜酸性粒细胞明显浸润现象;BALF中IL-2、IL-4、TNF-α与ET-1的表达均明显高于生理盐水对照组(P<0.05)。阿奇霉素则显著降低肺泡灌洗液中中性粒细胞、淋巴细胞以及嗜酸性粒细胞含量;明显改善支气管上皮脱落、杯状细胞增生,支气管周围嗜酸性粒细胞浸润现象;BALF中IL-2、IL-4、TNF-α与ET-1的表达也明显低于OVA模型大鼠(P<0.05)。结论:阿奇霉素通过调节Th1/Th2失衡对过敏性哮喘的气道炎症具有明显的治疗作用。
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| 关 键 词： | 阿奇霉素 过敏性 气道炎症 Th1/Th2失衡 |
Effect of Azithromycin on Ovalbumin-Induced Allergic Airway Inflammation and Regulation of Th1/Th2 Imbalance |
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| GUO Hu,TAN Xian-shu,LIU Li-tao and WAN Li-hong.Effect of Azithromycin on Ovalbumin-Induced Allergic Airway Inflammation and Regulation of Th1/Th2 Imbalance[J].Journal of Modern Clinical Medicine,2012,38(3):176-178. |
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| Authors: | GUO Hu TAN Xian-shu LIU Li-tao and WAN Li-hong |
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| Affiliation: | 1.Chengdu No.6 People’s Hospital,Sichuan Chengdu 610051,China;2.West China School of Preclinical and Forensic Medicine,Sichuan University,Sichuan Chengdu 610041,China) |
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| Abstract: | Objective:To investigate the effect of Azithromycin on ovalbumin-induced allergic airway inflammation and regulation of Th1/Th2 imbalance.Methods:40 male SD rats were randomly divided into Azithromycin group and dexamethasone group and saline group and asthma model group.Ovalbumin(OVA)/Al(OH)3 sensitization and OVA pulverization inhalation induced allergic airway inflammation model which was assessed by quantifying leukocytes in the airway,expression of multiple inflammatory mediators in the BALF;and there was histological examination in lung.Results:Azithromycin at the dose of 25 mg/kg had anti-inflammation properties in the model of allergic airway inflammation.Compared to treatment with saline,azithromycin attenuated the ovalbumin-dependent airway inflammation,included accumulation of neutrophils,lymphocytes and eosinophils,secretion of ET-1,IL-2,IL-4 and TNF-α,and mucus cell hyperplasia.Conclusion:Azithromycin play an important role in ovalbumin-induced allergic airway inflammation via regulation of Th1/Th2 imbalance. |
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| Keywords: | Azithromycin asthma inflammation Th1/Th2 imbalance |
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