| 荧光定量PCR检测戊肝病毒的应用价值探究 |
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| 引用本文： | 陈欣1,梁雨然2,曹誉龄1,李进春1,李佩菁1,曹昊1,杨颖馨1. 荧光定量PCR检测戊肝病毒的应用价值探究[J]. 现代预防医学, 2015, 0(22): 4162-4164 |
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| 作者姓名： | 陈欣1 梁雨然2 曹誉龄1 李进春1 李佩菁1 曹昊1 杨颖馨1 |
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| 作者单位： | 1.成都市锦江区疾病预防控制中心，四川 成都 610021；2.美国科罗拉多州立大学，科罗拉多州 科林斯堡 80526 |
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| 摘 要： | 摘要:目的 探讨荧光定量PCR检测HEV RNA的应用价值。方法 从110535份血清标本中，随机抽取标本200份，分别进行ELISA检验和荧光定量PCR分析。结果 69例ELISA阳性的标本经荧光定量PCR检测均为阳性，31例ELISA可疑的标本经荧光PCR检测也均为阳性，100例ELISA阴性的标本中有2例经荧光定量PCR检测结果为阳性。经Kappa检验，Kappa值为0.72，可认为2种方法检测结果具有中度一致性。采用Stuart-Maxwell检验，结果显示PCR与ELISA检出3种情况的边际概率不相等（χ2＝33，P＝0.00，P＜0.05），提示2种方法检测结果存在差异，即荧光PCR的阳性检出率更高。结论 荧光定量PCR检测HEV RNA能客观地反映HEV感染、复制及病程变化，修正或补充对ELISA测定结果的判定和解释，有利于HEV感染的早期诊断。
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| 关 键 词： | 关键词:荧光定量聚合酶链反应 酶联免疫吸附测定实验 戊型肝炎病毒RNA |
Exploration of the application value of fluorescent quantitative PCR on detection of hepatitis E virus |
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| CHEN Xin,LIANG Yu-ran,CAO Yu-ling,LI Jin-chun,LI Pei-jing,CAO Hao,YANG Ying-xin. Exploration of the application value of fluorescent quantitative PCR on detection of hepatitis E virus[J]. Modern Preventive Medicine, 2015, 0(22): 4162-4164 |
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| Authors: | CHEN Xin LIANG Yu-ran CAO Yu-ling LI Jin-chun LI Pei-jing CAO Hao YANG Ying-xin |
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| Affiliation: | *Jinjiang Center for Disease Control and Prevention, Chengdu, Sichuan 610021, China |
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| Abstract: | Abstract: Objective The objective of the study was to explore the application value of fluorescent quantitative PCR (FQ-PCR) on detection of hepatitis E virus (HEV) RNA. Methods 200 samples were randomly selected from 110535 serum specimens, and were analyzed ELISA and FQ-PCR. Results All 69 ELISA-positive, 31 ELISA-ambiguous, and 2 out of 100 ELISA-negative samples were tested positive of HEV RNA by FQ-PCR. The Kappa value was 0.72, indicating that results of the two methods were of moderate consistency. Stuart-Maxwell test showed that PCR and ELISA resulted in different marginal probabilities of the three conditions (χ2=33, P=0.00, P<0.05), suggesting that results of the two methods were statistically different, and that FQ-PCR had higher positive rate. Conclusion Detection of HEV RNA by FQ-PCR can objectively reflect the infection, replication, and course changes of HEV, and can correct or supplement the results of ELISA assays; therefore, is conducive to early diagnosis of HEV infection. |
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| Keywords: | Keywords: Fluorescent quantitative PCR (FQ-PCR) Enzyme-linked immunosorbent assay (ELISA) Hepatitis E virus (HEV) RNA |
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