Title:

Influence of cold stress on stress hormones and T lymphocyte cytokines in rats

作者：

周文芸¹, 杨思俊¹, 魏辉平¹, 文珠², 曾黎峰¹, 何丹¹, 胡国柱¹

周文芸¹，杨思俊¹，魏辉平¹，文珠²，曾黎峰¹，何丹¹，胡国柱¹

Author(s):

ZHOU Wen-yun*, YANG Si-jun, WEI Hui-ping, WEN Zhu, ZENG Li-feng, HE Dan, HU Guo-zhu

ZHOU Wen-yun*, YANG Si-jun, WEI Hui-ping, WEN Zhu, ZENG Li-feng, HE Dan, HU Guo-zhu

单位:

1.江西省人民医院，江西 南昌 330006；
2.江西省医学科学研究院血液研究室，江西 南昌 330006

Unit:

*Jiangxi people's hospital, Nanchang, Jinagxi 330006, China

关键词:

关键词：温度; 冷应激; 大鼠; 外周血; 激素; 脂多糖; 刀豆素-A; T淋巴细胞因子

关键词：温度；冷应激；大鼠；外周血；激素；脂多糖；刀豆素-A；T淋巴细胞因子

Keywords:

Keywords: Ambient temperature; Cold stress; Concanavalin-A; Hormones; Lipopolysaccharide; Peripheral blood; Rat

分类号:

R319

文献标识码:

A

摘要:

摘要：目的　探讨冷应激对大鼠外周血应激激素及T淋巴细胞因子的影响。方法　将230只SD大鼠分为20℃、4℃、-12℃ 3个环境温度组，每个温度组分非刺激、细菌脂多糖刺激、刀豆素-A刺激亚组，每亚组分1、12、24、48 h 4个时间点观察组；ELISA测定血浆促肾上腺皮质激素，肾上腺素，血管紧张素-2，白介素-2，干扰素-γ，白介素-4和白介素-10。结果　与20℃比较，大鼠4℃和-12℃冷应激血浆促肾上腺皮质激素（1 h，24.48±1.58比51.37±32.32和52.95±3.41；12 h，35.62±5.46比58.12±30.86和73.26±7.14）和肾上腺素（1 h，41.12±2.59比96.45±32.27和110.87±4.66；12 h，73.99±24.28比127.34±77.43和126.36±31.48）、大鼠-12℃冷应激血浆血管紧张素-2（1 h：118.84±31.41比437.13±71.72；12 h，318.08±93.44比440.72±69.43；24 h，247.11±97.79比620.83±67.45；48 h，171.99±67.72比556.38±94.62）以及大鼠冷应激血浆白介素-10（4℃、1 h，21.14±5.27比53.84±25.26；12 h，38.78±14.96比70.02±34.41；24 h，35.65±4.24比57.32±20.70；48 h，37.39±12.31比65.40±32.11；-12℃、1 h，比64.89±13.01，12 h，比68.33±7.31）水平显著升高（P＜0.05）、脂多糖和刀豆素-A刺激机体未改变这种趋势。与20℃比较，血浆IL-2水平在脂多糖刺激下-12℃（48 h，938.18±193.11比675.65±88.14）和刀豆素-A刺激下4℃（1～ 48 h，786.67±134.58比462.34±102.42；1014.07±164.34比472.70±83.88；1194.69±152.03比392.66±41.43；991.56±225.16比501.89±46.65）和-12℃（24 h，比874.58±143.38；48 h比640.24±99.24）、血浆IL-4水平在4℃（12 h，115.58±43.14比62.36±11.94；24 h，90.76±31.85比56.66±20.16；48 h，93.69±37.56比68.09±12.59）和-12℃（12 h，比73.73±11.16）、脂多糖刺激时IL-4水平在4℃（24 h，110.49±51.58比60.13±23.05）以及刀豆素-A刺激时IL-4水平在4℃（1 h，58.25±10.00比32.47±10.43；12 h，125.45±56.12比53.22±9.04；24 h，109.36±15.22比37.93±15.21）和-12℃（12 h，比70.36±9.79）、血浆IFN-γ水平脂多糖刺激时在4℃（12 h，1751.66±618.91比1483.62±390.59）和-12℃（48 h，1829.49±414.15比1184.42±461.01）以及刀豆素-A刺激时在4℃（24 h，1738.92±192.44比1106.13±324.29）显著降低（P＜0.05）。结论　气温骤降可致大鼠应激激素升高以及适应性免疫功能受损。

Abstract:

Abstract: Objective The aim of this study was to determine the influence of sudden ambient temperature drop on the levels of stress hormones and T lymphocyte cytokines in plasma. Methods Two hundred and thirty SD rats were divided into three groups in different ambient temperatures (20℃, 4℃ and -12℃). In each ambient temperature group, there were non-stimulation, lipopolysaccharide-stimulation and concanavalin-A-stimulation groups. In each group, there were four observation time points (1 h, 12 h, 24 h and 48 h). The levels of adrenocorticotropin, epinephrine, angiotensin-II, interleukin-2, interferon-γ, interleukin-4 and interleukin-10 in plasma were determined using ELISA. Results In rats exposed to cold stress, the levels of adrenocorticotropin at 1h were 24.48±1.58 at 20℃, 51.37±32.32 at 4℃ and 52.95±3.41 at -12℃, and the levels of adrenocorticotropin at 12h were 35.62±5.46 at 20℃, 58.12±30.86 at 4℃ and 73.26±7.14 at -12℃. The levels of epinephrine at 1h were 41.12±2.59 at 20℃, 96.45±32.27 at 4℃ and 110.87±4.66 at -12℃, and the levels of epinephrine at 12h were 73.99±24.28 at 20℃, 127.34±77.43 at 4℃ and 126.36±31.48 at -12℃. The levels of angiotensin-II at 1h were 118.84±31.41 at 20℃ and 437.13±71.72 at -12℃, the level of angiotensin-II at 12h were 318.08±93.44 at 20℃ and 440.72±69.43 at -12℃, the level of angiotensin-II at 24h were 247.11±97.79 at 20℃ and 620.83±67.45 at -12℃, the level of angiotensin-II at 48h were 171.99±67.72 at 20℃ and 556.38±94.62 at -12℃. The level of interleukin-10 at 1h were 21.14±5.27 at 20℃, 53.84±25.26 at 4℃ and 64.89±13.01 at -12℃, the level of interleukin-10 at 12h were 38.78±14.96 at 20℃, 70.02±34.41 at 4℃ and 68.33±7.31 at -12℃, the level of interleukin-10 at 24h were 35.65±4.24 at 20℃ and 57.32±20.70 at 4℃, and the level of interleukin-10 at 48h were 37.39±12.31 at 20℃ and 65.40±32.11 at 4℃ in plasma, which were significantly elevated compared to rats at 20℃ (P<0.05). Similar effects were observed in lipopolysaccharide or concanavalin-A-stimulated rats exposed to cold stress. This tendency was not significantly changed. In rats exposed to cold stress, the levels of interleukin-4 at 12h were 115.58±43.14 at 20℃, 62.36±11.94 at 4℃ and 73.73±11.16 at -12℃, the levels of interleukin-4 at 24h were 90.76±31.85 at 20℃ and 56.66±20.16 at 4℃, and the levels of interleukin-4 at 48h were 93.69±37.56 at 20℃ and 68.09±12.59 at 4℃ in plasma, which were significantly reduced compared to rats at 20℃ (P<0.05). The levels of interleukin-2 at 48h were 938.18±193.11 at 20℃ and 675.65±88.14 at -12℃ in lipopolysaccharide-stimulated rats, and the levels of interleukin-2 at 1h were 786.67±134.58 at 20℃ and 462.34±102.42 at 4℃, the levels of interleukin-2 at 12h were 1014.07±164.34 at 20℃ and 472.70±83.88 at 4℃, the levels of interleukin-2 at 24h were 1194.69±152.03 at 20℃, 392.66±41.43 at 4℃ and 874.58±143.38 at -12℃, and the levels of interleukin-2 at 48h were 991.56±225.16 at 20℃, 501.89±46.65 at 4℃ and 640.24±99.24 at -12℃ in concanavalin-A-stimulated rats. The levels of interleukin-4 at 24h were 110.49±51.58 at 20℃ and 60.13±23.05 at 4℃ in lipopolysaccharide-stimulated rats, and the levels of interleukin-4 at 1h were 58.25±10.00 at 20℃ and 32.47±10.43 at 4℃, the levels of interleukin-4 at 12h were 125.45±56.12 at 20℃, 53.22±9.04 at 4℃ and 70.36±9.79 at -12℃, and the levels of interleukin-4 at 24h were 109.36±15.22 at 20℃ and 37.93±15.21 at 4℃ in concanavalin-A-stimulated rats. And the levels of interferon-γ at 12h were 1751.66±618.91 at 20℃ and 1483.62±390.59 at 4℃ and the levels of interferon-γ at 48h were 1829.49±414.15 at 20℃ and 1184.42±461.01 at -12℃ in lipopolysaccharide-stimulated rats, and the levels of interferon-γ at 24h were 1738.92±192.44 at 20℃ and 1106.13±324.29 at 4℃ in concanavalin-A-stimulated rats, which were significantly reduced compared to rats at 20℃ (P<0.05). Conclusion These data indicated that cold stress affected the stress hormones and the adaptive immunity functions in rats.

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备注/Memo:

基金项目： 国家科技部“十一五”科技支撑计划（项目编号：2008Bai68b01）
作者简介： 周文芸（1971-），女，大专，主管技师，研究方向：免疫学
通讯作者： 胡国柱，E-mail：hgz56@126.com