| p75神经营养蛋白受体阳性舌鳞状细胞癌细胞的生物学特性研究 |
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| 引用本文： | 佟冬冬,张风河,姚瑶,张兆弢,王进兵,李青,张新莲.p75神经营养蛋白受体阳性舌鳞状细胞癌细胞的生物学特性研究[J].华西口腔医学杂志,2014,32(1):18-22. |
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| 作者姓名： | 佟冬冬 张风河 姚瑶 张兆弢 王进兵 李青 张新莲 |
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| 作者单位： | 1.山东大学口腔医院口腔颌面外科；2.山东省口腔生物医学重点实验室，济南 250012；3.安徽省第二人民医院口腔科，合肥 230022 |
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| 基金项目： | 山东省科学技术发展计划基金资助项目(项目编号：2010G00202-44) |
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| 摘 要： | 目的 对流式细胞仪分选获得的p75神经营养蛋白受体（p75NTR）阳性舌鳞状细胞癌细胞进行生物学特性研究。方法 通过流式细胞术检测舌鳞状细胞癌细胞系Tca-8113和Cal-27中p75NTR阳性细胞的表达。对流式细胞仪分选获取的 p75NTR阳性细胞进行单克隆培养、四甲基偶氮唑盐比色法及划痕实验检测，以未分选细胞为对照，分析 p75NTR阳性细胞的生物学特性。结果 舌鳞状细胞癌细胞株Tca-8113、Cal-27中，p75NTR阳性细胞的比例分别为3.1％和1.9％。与未分选的细胞相比，p75NTR阳性细胞的单克隆形成能力更高（ Tca-8113细胞， P=0.024；Cal-27细胞，P=0.009）；单克隆 p75NTR阳性细胞再培养 2周后， p75NTR阳性细胞率分别降为14.5%（Tca-8113）和5.8%（Cal-27）；p75NTR阳性细胞体外增殖能力显著增强，且具有明显的侵袭迁移能力。结论 p75NTR阳性舌鳞状细胞癌细胞具有肿瘤干细胞的特性。
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| 关 键 词： | 舌鳞状细胞癌 肿瘤干细胞 p75神经营养蛋白受体 |
Study of the biological characteristics of p75 neurotrophin receptor positive tongue squamous cell carcinoma cells |
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| Tong Dongdong,Zhang Fenghe,Yao Yao,Zhang Zhaotao,Wang Jinbing,Li Qing,Zhang Xinlian.Study of the biological characteristics of p75 neurotrophin receptor positive tongue squamous cell carcinoma cells[J].West China Journal of Stomatology,2014,32(1):18-22. |
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| Authors: | Tong Dongdong Zhang Fenghe Yao Yao Zhang Zhaotao Wang Jinbing Li Qing Zhang Xinlian |
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| Affiliation: | 1. Dept. of Oral and Maxillofacial Surgery, Stomatology Hospital of Shandong University, Jinan 250012, China;2. Shandong Provincial Key Labo-ratory of Oral Biomedicine, Jinan 250012, China; 3. Dept. of Stomatology, Anhui No.2 Provincial People’s Hospital, Anhui 230022, China |
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| Abstract: | Objective To study the biological characteristics of p75 neurotrophin receptor positive (p75NTR+) tongue squa-mous cell carcinoma cells which were separated by flow cytometry cell sorting. Methods To determine the biological cha-racteristics of p75NTR+ cells which were separated from Tca-8113 and Cal-27 tongue squamous cell carcinoma cells by flow cytometry cell sorting, including study the capacity of cloning, 3-(4,5)-demethylthiazo(z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, wound healing assay. p75NTR+ cells with non-sorted cells were as control group. Results In Tca-8ll3 and Cal-27 tongue squamous cell carcinoma cell lines, the percentage of p75NTR+ cells were 3.1％ and 1.9％. Compared with p75NTR+ cells with non-sorted cells, p75NTR+ cells possess higher capacity of cloning (Tca-8113, P=0.024; Cal-27, P=0.009). The per-centage of p75NTR+cells of the progeny cells generated from monoclonal p75NTR+ cells decreased to 14.5％ (Tca-8113) and 5.8％ (Cal-27) after cultured two weeks. p75NTR+ cells possessed higher proliferation ability and higher metastasis ability than non-sorted cells. Conclusion p75NTR+ cells isolated from tongue squamous cell carcinoma have the characteristics of cancer stem cells. |
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| Keywords: | tongue squamous cell carcinoma cancer stem cells p75 neurotrophin receptor |
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