| 基于 NF-κB 通路探讨绿萝花黄酮治疗 DPN 小鼠的机制 |
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| 引用本文： | 陈明霞,马雪,陈佩文,袁慧,冷伟.基于 NF-κB 通路探讨绿萝花黄酮治疗 DPN 小鼠的机制[J].医学分子生物学杂志,2022,19(6):437-445. |
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| 作者姓名： | 陈明霞 马雪 陈佩文 袁慧 冷伟 |
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| 作者单位： | 1陕西中医药大学 陕西省咸阳市, 712000
2陕西中医药大学附属医院风湿血液免疫科 陕西省咸阳市, 712000 |
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| 基金项目： | 陕西省自然科学基础研究计划项目(No. 2018JQ8061) |
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| 摘 要： | 目的 基于 NF-κB 通路探讨绿萝花黄酮治疗 DPN 小鼠的机制。 方法 将 60 只小鼠分为对照组、
模型组、 绿萝花黄酮低、 中和高剂量组、 阿卡波糖组各 10 只。 将小鼠雪旺细胞分为正常组、 LPS 组、 绿萝
花黄酮低、 中、 高剂量组。 比较各组小鼠神经传导功能指标、 热痛阈值、 炎性因子、 NF-κB 信号通路关键
蛋白及相关 mRNA 表达水平。 结果 绿萝花黄酮提高小鼠 MNCV、 SNCV 及热痛阈值, 降低炎性因子水平
(P < 0. 05); 降低 p-NF-κB P65 / NF-κB P65、 p-IKKβ/ IKKβ 蛋白比值, 提高 p-IκBα/ IκBα 蛋白比值 ( P <
0. 05); 下调 NF-κB P65、 IKKβ mRNA, 上调 IκBα mRNA (P < 0. 05)。 结论 绿萝花黄酮通过抑制 NF-κB
信号通路降低炎性因子释放, 减轻 DPN 小鼠病情。
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| 关 键 词： | 核因子 κB 糖尿病周围神经病变 绿萝花黄酮 小鼠 |
Mechanism of Flavonoids from Scindapsus Aureus in the Treatment of
DPN Mice Based on NF-κB Pathway |
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| CHEN Mingxia,MA Xue,CHEN Peiwen,YUAN Hui,LENG Wei.Mechanism of Flavonoids from Scindapsus Aureus in the Treatment of
DPN Mice Based on NF-κB Pathway[J].Journal of Medical Molecular Biology,2022,19(6):437-445. |
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| Authors: | CHEN Mingxia MA Xue CHEN Peiwen YUAN Hui LENG Wei |
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| Affiliation: | 1
Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi, 712000, China
2 Department of Rheumatology Blood Immunology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi, 712000, China |
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| Abstract: | Objective To explore the mechanism of flavonoids from scindapsus aureus in the
treatment of DPN mice via NF-кB pathway. Methods A total of 60 mice were divided into 6 groups
as follows, the control group, the model group, the low-dose, medium-dose and high-dose flavonoid groups, and the acarbose group, 10 cases per group. Schwann cells were divided into 5
groups: the normal group, the LPS group, and the low-dose, medium-dose and high-dose flavonoid groups. The nerve conduction function indexes, thermal pain threshold, levels of inflammatory factors, expression levels of NF-кB signaling pathway key proteins and their related mRNA
were compared among different groups. Results Flavonoids from scindapsus aureus could increase
the MNCV, the SNCV and the thermal pain threshold in mice, and reduce the levels of inflammatory factors (P< 0. 05). The flavonoid could decrease the ratios of p-NF-кB P65 / NF-кB P65 and p-IKKβ / IKKβ, and increase the p-IкBα / IκBα ratio (P< 0. 05). It could also down-regulate the expression levels of NF-кB P65 and IKKβ mRNA, and up-regulate the expression level of IкBα mRNA (P < 0. 05). Conclusion Flavonoids from scindapsus aureus can reduce the levels of inflammatory factors and relieve the symptoms in the DPN mice by inhibiting NF-κB signaling pathway. |
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| Keywords: | nuclear factor kappa B diabetic peripheral neuropathy flavonoids from scindapsus aureus mouse |
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