| 细胞角蛋白 19 通过调控 MAPK 和 PTEN 通路促进甲状腺癌细胞
的增殖、 侵袭及转移 |
| |
| 引用本文： | 张强,霍占江,田亮,王志杰,郝敏丽.细胞角蛋白 19 通过调控 MAPK 和 PTEN 通路促进甲状腺癌细胞
的增殖、 侵袭及转移[J].医学分子生物学杂志,2022,19(5):399-402. |
| |
| 作者姓名： | 张强 霍占江 田亮 王志杰 郝敏丽 |
| |
| 作者单位： | 张家口市第一医院耳鼻喉头颈外科 河北省张家口市, 075000 |
| |
| 摘 要： | 目的 探究细胞角蛋白 19 对甲状腺癌细胞生物学过程的影响及相关机制。 方法 将人甲状腺癌
细胞 (human thyroid cancer cells, B-CPAP) 作为研究对象, 使用不同浓度的角蛋白 19 处理细胞, 通过 MTT
实验、 细胞划痕实验和肿瘤细胞侵袭实验探究细胞角蛋白 19 对 B-CPAP 增殖、 侵袭及转移的影响。 最后通
过研究相关蛋白分析细胞角蛋白 19 对 B-CPAP 的作用机制。 结果 MTT 实验发现, 随着角蛋白 19 浓度升
高, 其对 B-CPAP 的增殖率也明显提高。 Transwell 实验发现, 与空白对照组比较, 角蛋白 19 处理后通过小
室的细胞数明显增加。 细胞划痕实验发现, 角蛋白 19 能够促进 B-CPAP 的迁移能力, 并且 B-CPAP 的迁移
能力与角蛋白 19 浓度呈正相关。 此外, 角蛋白 19 的这一作用与 PTEN 和 MAPK 通路有关。 结论 细胞角
蛋白 19 能够明显提高 B-CPAP 增殖、 侵袭以及转移的能力, 同时角蛋白 19 的这一作用与 PTEN 和 MAPK 通
路有关。
|
| 关 键 词： | 细胞角蛋白 19 甲状腺肿瘤 增殖 侵袭 转移 |
Effect of Cytokeratin 19 on the Proliferation,Invasion and Metastasis
of Thyroid Cancer Cells |
| |
| ZHANG Qiang,HUO Zhanjiang,TIAN Liang,WANG Zhijie,HAO Mingli.Effect of Cytokeratin 19 on the Proliferation,Invasion and Metastasis
of Thyroid Cancer Cells[J].Journal of Medical Molecular Biology,2022,19(5):399-402. |
| |
| Authors: | ZHANG Qiang HUO Zhanjiang TIAN Liang WANG Zhijie HAO Mingli |
| |
| Affiliation: | Department of Otolaryngology, Head and Neck Surgery of Zhangjiakou First Hospital, Zhangjiakou,
Hebei, 075000 |
| |
| Abstract: | Objective To explore the effect of cytokeratin 19 on the proliferation, invasion and
metastasis of thyroid cancer cells and the related mechanisms. Methods In this study, human thyroid cancer cells (B-CPAP) were used as the research object. MTT assay, wound healing assay
and cell invasion assay were applied to investigate the effect of cytokeratin 19 on the cell proliferation, invasion and metastasis after the treatment of keratin 19 on B-CPAP. Western blotting was used
to detect the related protein expressions to explore the underlying mechanisms. Results MTT assay
showed that the proliferation rate of B-CPAP increased significantly with the increasing concentration
of keratin 19 treatment. Transwell assay and wound healing assay results showed that keratin 19 can
promote the migration ability of B-CPAP in a dose-dependent manner significantly. In addition,
PTEN and MAPK pathways may play roles in that process. Conclusion Cytokeratin 19 can significantly increase the proliferation, invasion and metastasis of B-CPAP, and this effect of keratin 19 is
related to PTEN and MAPK pathways. |
| |
| Keywords: | cytokeratin 19 thyroid neoplasms proliferation invasion metastasis |
|
| 点击此处可从《医学分子生物学杂志》浏览原始摘要信息 |
|
点击此处可从《医学分子生物学杂志》下载全文 |
|
