| 猪囊尾蚴线粒体NADH脱氢酶的基因克隆 |
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| 引用本文： | 张莉,刘殿武.猪囊尾蚴线粒体NADH脱氢酶的基因克隆[J].中国寄生虫学与寄生虫病杂志,2003,21(1):34-36. |
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| 作者姓名： | 张莉 刘殿武 |
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| 作者单位： | 河北医科大学流行病学教研室,石家庄,050017 |
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| 基金项目： | 河北省自然科学基金 (No.3972 62 )~~ |
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| 摘 要： |
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| 关 键 词： | 猪囊尾蚴 cDNA文库 NADH脱氢酶 |
| 文章编号： | 1000-7423(2003)-01-0034-03 |
| 修稿时间： | 2002年1月30日 |
Cloning and Characterization of Mitochondrial NADH Dehydrogenase Gene of Cysticercus cellulosae |
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| ZHANG Li,LIU Dian-wu.Cloning and Characterization of Mitochondrial NADH Dehydrogenase Gene of Cysticercus cellulosae[J].Chinese Journal of Parasitology and Parasitic Diseases,2003,21(1):34-36. |
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| Authors: | ZHANG Li LIU Dian-wu |
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| Affiliation: | Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017. |
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| Abstract: | OBJECTIVE: To clone and characterize the NADH1 gene of Cysticercus cellulosae. METHODS: A cDNA library was constructed from Cysticercus cellulosae and was immunoscreened by using rabbit anti-Cysticercus cellulosae polyclonal antibody. The gene structure and its possible function were analyzed by comparing with sequences available in the GenBank, after the insert of positive clone was subcloned and the nucleotide sequence of the insert was determined by dideoxynucleotide chain termination method. RESULTS AND CONCLUSION: A cDNA clone (named TS5) with a length of 1,082 bp was isolated. The 5' terminal of cloned gene contained one open reading frame of 1-578 bp encoding 192 amino acid residues of mitochondrial NADH dehydrogenase subunit 1 and the 3' terminal contained three kinds of tRNA genes. |
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| Keywords: | Cysticercus cellulosae cDNA library NADH dehydrogenase |
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