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微小隐孢子虫DNA探针的制备
引用本文:郭步平,张建斌,连德润. 微小隐孢子虫DNA探针的制备[J]. 中国寄生虫学与寄生虫病杂志, 1999, 17(2): 113-114
作者姓名:郭步平  张建斌  连德润
作者单位:长治医学院寄生虫学教研室,中山医科大学寄生虫病研究所
摘    要:目的:制备一种高度特异敏感的隐孢子虫检测探针。方法:应用聚合酶链反应(PCR)扩增微小隐孢子虫的一段核苷酸片段,扩增产物452bpDNA用半抗原地高辛标记制备成探针。结果:经敏感性试验,该探针可检测2pg水平的隐孢子虫DNA。用该探针与隐孢子虫DNA和溶组织内阿米巴、贾第虫、大肠杆菌及痢疾杆菌等相关生物的DNA和隐孢子虫宿主的DNA进行斑点杂交试验,该探针只与隐孢子虫DNA杂交。结论:该探针具有高度特异性和较高敏感性。

关 键 词:微小隐孢子虫  DNA探针  杂交

PREPARATION OF DNA PROBE FOR CRYPTOSPORIDIUM PARVUM
GUO Buping,ZHANG Jianbin,LIAN Derun. PREPARATION OF DNA PROBE FOR CRYPTOSPORIDIUM PARVUM[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1999, 17(2): 113-114
Authors:GUO Buping  ZHANG Jianbin  LIAN Derun
Affiliation:Department of parasitology, Chagzhi Medical College, Changzhi 046000.
Abstract:AIM: To prepare a probe with high specificity and sensitivity for the detection of Cryptosporidium parvum. METHODS: Using PCR method, a fragment from the DNA of C.parvum was amplified . The PCR product, 452 bp DNA, was labeled with hapten digoxigenin.RESULTS: Examination of sensitivity showed that the DNA probe could detect as low as 2 pg DNA from C.parvum. The dot blot hybridizition assay showed that the probe hybridized with the DNA of C.parvum, but not hybridized with DNA of E.histolytica, G.lamblia, E.coli and D.bacilli. CONCLUSION: The probe was highly specific and sensitive for the detection of C.parvum.
Keywords:Cryptosporidium parvum   DNA probe   hybridization
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