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Detection of Chlamydia trachomatis,Ureaplasma urealyticum and Mycoplasma hominis in infertile Bulgarian men with multiplex real‐time polymerase chain reaction
Authors:Vessela Ouzounova‐Raykova  Simeon Rangelov  Iordanka Ouzounova  Ivan Mitov
Affiliation:1. Department of Medical Microbiology, Medical University‐Sofia, Sofia, Bulgaria;2. Department of Andrology, University Hospital ‘Tsaritsa Yoanna’, Medical University‐Sofia, Sofia, Bulgaria
Abstract:The effect of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis over the sperm quality is still unclear. The aim of this study was to determine their prevalence in infertile Bulgarian men. A total of 281 men were examined by applying mRT‐PCR. The registered prevalence was as follows: C. trachomatis – 13.9%, U. urealyticum – 19.2%, M. hominis – 9.9%. Co‐infection was established in eight swabs. This first in Bulgaria to study for detection of chlamydia and mycoplasmas in infertile men by mRT‐PCR demonstrates higher prevalence of the tested microorganisms in the infertile group toward the control one.
Keywords:Chlamydia  Mycoplasmas  mRT‐PCR  prevalence  infertile men
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