| 重组人骨形成蛋白2对人牙周膜成纤维细胞的生物学作用 |
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| 引用本文： | 司晓辉,刘正. 重组人骨形成蛋白2对人牙周膜成纤维细胞的生物学作用[J]. 华西口腔医学杂志, 2002, 20(1): 10-12 |
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| 作者姓名： | 司晓辉 刘正 |
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| 作者单位： | 200011,上海第二医科大学附属第九人民医院口腔医学研究所;200011,上海第二医科大学附属第九人民医院口腔医学研究所 |
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| 基金项目： | 国家自然科学基金资助项目 (编号 3 0 0 0 0 191) |
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| 摘 要： | 目的:研究重组人骨形成蛋白2 (rhBMP2) 对人牙周膜成纤维细胞(HPDLFs) 的生物学作用。方法:原代培养HPDLFs ,并观察rhBMP2 对HPDLFs 增殖、碱性磷酸酶(ALP) 活性、骨钙素(OC) 合成及矿化结节形成的作用。结果:0125～2 mgPml rhBMP2 对HPDLFs 的增殖无明显作用,015～2 mgPml rhBMP2 显著提高HPDLFs 的ALP 活性,刺激OC合成和矿化结节形成。结论:rhBMP2 对HPDLFs 的作用具有剂量依赖性。rhBMP2 能够刺激HPDLFs 表达成骨细胞表型并促进其表型成熟。
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| 关 键 词： | 重组人骨形成蛋白2 人牙周膜成纤维细胞 碱性磷酸酶 骨钙素 矿化 |
| 收稿时间： | 2002-02-25 |
| 修稿时间： | 2000-03-20 |
The Biological Effects of Recombinant Human Bone Morphogenetic Protein 2 on Human Periodontal Ligament Fibroblasts |
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| Xiaohui Si,Zheng Liu. The Biological Effects of Recombinant Human Bone Morphogenetic Protein 2 on Human Periodontal Ligament Fibroblasts[J]. West China journal of stomatology, 2002, 20(1): 10-12 |
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| Authors: | Xiaohui Si Zheng Liu |
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| Affiliation: | The Ninth People′s Hospital , Shanghai Second Medical University |
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| Abstract: | OBJECTIVE: The purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs). METHODS: Human periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules. RESULTS: rhBMP2 (0.25-2 mg/ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 0.5-2 mg/ml rhBMP2. CONCLUSION: The effects of rhBMP2 on HPDLFs are dose-dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs. |
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| Keywords: | recombinant human bone morphogenetic protein 2 human periodontal ligament fibroblasts alkaline phosphatase osteocalcin mineralization |
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