| 神经型钙黏蛋白分子在多发性骨髓瘤患者中的表达 |
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| 引用本文： | 王瑞,李振宇,李德鹏,闫志凌,张之尧,陈丽丽,吴庆运,陈翀,潘秀英.神经型钙黏蛋白分子在多发性骨髓瘤患者中的表达[J].白血病．淋巴瘤,2014,23(8):456-459. |
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| 作者姓名： | 王瑞 李振宇 李德鹏 闫志凌 张之尧 陈丽丽 吴庆运 陈翀 潘秀英 |
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| 作者单位： | 221002,徐州医学院附属医院血液科 |
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| 摘 要： | 目的 探讨神经型钙黏蛋白（N-cadherin）分子在多发性骨髓瘤（MM）患者中的表达及其临床意义.方法 收集54例初治MM患者及20名健康人骨髓单个核细胞,应用实时荧光定量PCR法检测N-cadherin mRNA表达,Western blot法测定其蛋白表达.结果 MM患者N-cadherin mRNA表达水平为6.056±3.033,健康对照组为1.788±0.778,二组相比差异有统计学意义（P＜0.0001）.按照ISS分期,Ⅰ期、Ⅱ期、Ⅲ期MM患者N-cadherin mRNA表达水平分别为3.681±3.185、5.757±3.292、7.460±3.647,其中Ⅱ期、Ⅲ期与健康对照组相比差异均具有统计学意义（均P＜0.05）;Ⅰ期与健康对照组相比差异无统计学意义（P＞0.05）.有髓外浸润及无髓外浸润患者N-cadherin mRNA表达量分别为9.159±3.178、5.083±3.288,差异有统计学意义（P＜ 0.001）.有骨质破坏和无骨质破坏患者N-cadherin mRNA表达量分别为6.544±3.729、4.240±2.896,差异有统计学意义（P=0.047）.初治MM患者N-cadherin mRNA表达水平为6.056±3.033,治疗后有效患者N-cadherin mRNA表达水平下降（3.768±2.216）（P=0.015）,治疗无效患者N-cadherin mRNA水平与治疗前相比差异无统计学意义（P＞0.05）.初治MM患者N-cadherin蛋白表达量明显高于缓解期患者及健康对照组,而在治疗后复发的患者中,N-cadherin蛋白表达量明显高于初治患者.结论 N-cadherin分子在MM患者中高表达,其可能参与MM的发生及发展.
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| 关 键 词： | 多发性骨髓瘤 神经型钙黏蛋白 |
Expression of N-cadherin in patients with multiple myeloma |
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| Wang Rui,Li Zhenyu,Li Depeng Yah Zhiling,Zhang Zhiyao,Chen Lili,Wu Qingyun,Chen Chong,Pan Xiuying,Xu Kailin.Expression of N-cadherin in patients with multiple myeloma[J].Journal of Leukemia & Lymphoma,2014,23(8):456-459. |
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| Authors: | Wang Rui Li Zhenyu Li Depeng Yah Zhiling Zhang Zhiyao Chen Lili Wu Qingyun Chen Chong Pan Xiuying Xu Kailin |
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| Affiliation: | . (Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China) |
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| Abstract: | Objective To investigate the expression and clinical significance of N-cadherin in patients with multiple myeloma （MM）.Methods A total of newly diagnosed 54 MM patients and 20 controls were enrolled in this study.The expression of N-cadherin was detected by quantitative real-time polymerase chain reaction and Western blot.Results The N-cadherin mRNA expression levels of MM patients and normal controls were 6.056±3.033 and 1.788±0.778 （P 〈 0.000 1）.N-cadherin mRNA expression levels of ISS stage Ⅰ,Ⅱ and Ⅲ were 3.681±3.185,5.757±3.292 and 7.460±3.647,respectively.The results indicated that N-cadherin was significantly up-regulated in stage Ⅱ and stage Ⅲ （P 〈 0.05）,not in stage Ⅰ （P 〉 0.05） compared with controls.The median levels of N-cadherin mRNA expression in MM patients with or without extramedullary infiltration were 9.159±3.178 and 5.083±3.288,respectively （P 〈 0.001）.MM patients with bone destruction had a higher N-cadherin mRNA expression level （6.544±3.729） than patients without bone destruction （4.240±2.896） （P =0.047）.N-cadherin mRNA expression in newly diagnosed MM patients was 6.056±3.033,the expression in responded patients after treatment was significantly decreased （3.768±2.216） （P =0.015）,and in no responded patients the levels of N-cadherin were not significantly decreased （P 〉 0.05）.The levels of N-cadherin protein were up-regulated in newly diagnosed patients and relapsed patients compared with controls,which was reduced in remission MM patients.And relapsed patients had a higher N-cadherin protein expression than newly diagnosed patients.Conclusion High expression of N-cadherin in MM patients may play an important role during the occurrence and development of MM. |
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| Keywords: | Mutiple myeloma N-cadherin |
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