| 青藏高原岩羊朊蛋白基因及氨基酸序列分析 |
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| 引用本文： | 伍晶星,高利萍,陈冬冬,肖康,武月章,董小平,石琦.青藏高原岩羊朊蛋白基因及氨基酸序列分析[J].疾病监测,2022,37(2):167-171. |
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| 作者姓名： | 伍晶星 高利萍 陈冬冬 肖康 武月章 董小平 石琦 |
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| 作者单位： | 中国疾病预防控制中心病毒病预防控制所，传染病预防控制国家重点实验室，北京 102206 |
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| 基金项目： | 传染病预防控制国家重点实验室课题;国家重点研发计划 |
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| 摘 要： | 目的 分析岩羊的朊蛋白基因(PRNP)序列,评估岩羊对羊瘙痒病的易感性.方法 参照GenBank上已经发表的绵羊PRNP序列设计引物,对岩羊的PRNP序列进行扩增,基因测序后与GenBank上的11种哺乳动物的PRNP序列比对和分析.结果 测序结果显示岩羊PRNP由771个核苷酸构成,编码256个氨基酸.序列分析结果显...
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| 关 键 词： | 岩羊 羊瘙痒病 朊蛋白基因 多态性 序列分析 |
| 收稿时间： | 2021-01-27 |
Sequences of prion protein gene and amino acid of blue sheep on Qinghai-Tibet Plateau |
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| Wu Jingxing,Gao Liping,Chen Dongdong,Xiao Kang,Wu Yuezhang,Dong Xiaoping,Shi Qi.Sequences of prion protein gene and amino acid of blue sheep on Qinghai-Tibet Plateau[J].Disease Surveillance,2022,37(2):167-171. |
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| Authors: | Wu Jingxing Gao Liping Chen Dongdong Xiao Kang Wu Yuezhang Dong Xiaoping Shi Qi |
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| Affiliation: | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: | Objective To analyze the prion protein gene (PRNP) sequence of blue sheep and evaluate the susceptibility of blue sheep to scrapie. Methods The primers were designed according to the PRNP sequence of sheep published on GenBank, and then the PRNP sequence of blue sheep was amplified, sequenced, and compared with the PRNP sequence of 11 species of mammals published on GenBank. Results Gene sequencing results showed that the PRNP of blue sheep consists of 771 nucleotides and encodes 256 amino acids. Sequence analysis showed that the amino acid sequences of prion protein (PrP) in four blue sheep were completely homologous, and were consistent with those of wild-type sheep. In addition, the amino acid sequence of PrP in blue sheep showed high homology with goat (99.2%), reindeer (98.0%) and cattle (97.7%). Conclusion Four blue sheep enrolled in this study might be susceptible to scrapie as wild-type sheep, and have the potential to transmit scrapie between blue sheep and sheep. |
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