| 天麻素通过调节miR-155介导的Notch通路对脑卒中大鼠发挥神经保护作用 |
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| 引用本文： | 刘振,张磊,胡灿芳,罗国君,汤定中.天麻素通过调节miR-155介导的Notch通路对脑卒中大鼠发挥神经保护作用[J].国际医药卫生导报,2022,28(1):2-7. |
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| 作者姓名： | 刘振 张磊 胡灿芳 罗国君 汤定中 |
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| 作者单位： | 上海市第六人民医院金山分院神经内科，上海 201599 |
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| 基金项目： | 上海市金山区卫生健康委员会科研课题面上项目（JSKJ-KTMS-2019-07） |
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| 摘 要： | 目的:探究天麻素对脑卒中大鼠神经的保护作用及其机制。方法:2020年1月至2021年3月，将体质量为（250±10）g的清洁级健康雄性SD大鼠94只分为假手术组、模型组、天麻素低剂量组（50 mg/kg）、天麻素中剂量组（100 mg/kg）、天麻素高剂量组（150 mg/kg）。对大鼠神经功能进行Longa评分；称量...
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| 关 键 词： | 天麻素 miR-155 Notch通路 脑卒中大鼠 神经功能 |
| 收稿时间： | 2021-07-29 |
Gastrodin exerts neuroprotective effect on stroke rats by regulating miR-155-mediated Notch pathway |
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| Liu Zhen,Zhang Lei,Hu Canfang,Luo Guojun,Tang Dingzhong.Gastrodin exerts neuroprotective effect on stroke rats by regulating miR-155-mediated Notch pathway[J].International Medicine & Health Guidance News,2022,28(1):2-7. |
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| Authors: | Liu Zhen Zhang Lei Hu Canfang Luo Guojun Tang Dingzhong |
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| Affiliation: | Department
of Neurology, Jinshan Branch, Shanghai Sixth People's Hospital, Shanghai
201599, China |
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| Abstract: | Objective:To explore the protective effect of gastrodin on the nerves of stroke rats and its mechanism.Methods:From January 2020 to March 2021, 94 healthy clean SD rats whose body weight were (250±10) g were divided into a sham operation group, a model group, a low-dose gastrodin group (50 mg/kg), a medium-dose gastrodin group (100 mg/kg), and a high-dose gastrodin group (150 mg/kg). The rats' nerve function was scored by Longa; the brain water content was calculated by weighing; 2, 3, 5-triphenyltetrazolium chloride (TTC) staining was used to observe the cerebral infarction; the morphology of neurons in hippocampus was observed by hematoxylin eosin (HE) staining; the activities of Caspase-3 and caspase-9 were determined by colorimetry; real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of microRNA-155 (miR-155); and Western blot (WB) was used to detect the expression of Notch1 and its ligand Jagged1. The measurement data of normal distribution were expressed as (
xˉ±s), and were compared between 3 or more than 3 groups by one-way ANOVA and by SNK-q test between two groups. If
P<0.05, there is a statistical difference.
Results:Compared with those in the sham operation group, the neurological function in the model group was seriously damaged; the brain water content, cerebral infarction rate, and activities of Caspase-3 and caspase-9 were significantly increased; the expression of miR-155 in the brain was significantly increased; and there were no statistical differences in the expressions of Notch1, Jagged1, and Hes1 (all
P>0.05). Compared with those in the model group, the neurological function in the low, medium, and high dose gastrodin groups was reduced; the brain water content (81.52±2.19)%, (80.39±2.24)%, and (79.33±2.05)% vs. (87.44±2.56)%], cerebral infarction rate (24.66±2.35)%, (20.87±2.06)%, and (15.65±1.54)% vs. (32.15±3.59)%], and activities of Caspase-3 (2.48±0.31), (2.12±0.27), and (1.43±0.21) vs (3.27±0.42)] and Caspase-9 (2.05±0.25), (1.76±0.20), and (1.23±0.16) vs. (2.79±0.36)] were significantly decreased, the expression of miR-155 (2.15±0.18), (1.97±0.16), and (1.63±0.15) vs. (2.64±0.27)] in the brain was significantly decreased, and the expressions of Notch1 (0.77±0.13), (0.84±0.16), and (0.95±0.18) vs. (0.42±0.09)], Jagged1 (0.68±0.10), (0.73±0.15), and (0.87±0.16) vs. (0.38±0.06)], and Hes1 (0.81±0.12), (0.93±0.15), and (1.07±0.17) vs. (0.43±0.07)] were significantly increased.
Conclusion:Gastrodin can down-regulate the expression of miR-155 and activate Notch pathway, and protects the nerves of stroke rats. |
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| Keywords: | Gastrodin miR-155 Notch pathway Stroke rats Neurological function |
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