|
|||||
|
|
| 抗环子孢子蛋白保守II+区单克隆抗体的制备及鉴定 | |
| 引用本文： | 张瑞娟,朱淮民,李翔宇. 抗环子孢子蛋白保守II+区单克隆抗体的制备及鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2004, 2(2): 83-85 |
| 作者姓名： | 张瑞娟 朱淮民 李翔宇 |
| 作者单位： | 张瑞娟（第二军医大学病原生物学教研室,上海,200433） 朱淮民（第二军医大学病原生物学教研室,上海,200433） 李翔宇（第二军医大学病原生物学教研室,上海,200433） |
| 摘 要： | 目的 获得针对恶性疟原虫环子孢子蛋白 (CSP)保守II+ 区 (RegionII+ )的单克隆抗体。 方法 采用恶性疟原虫保守II+ 区十二肽 (EWSPCSVTCGNG)免疫BALB/c小鼠 ,经融合 ,ELISA 3次筛选 ,获得 3株分泌针对保守II+ 区单克隆抗体的杂交瘤细胞株。 结果 ELISA检测结果显示单抗能与重组表达的恶性疟原虫CSP片段及天然CSP特异性反应。间接荧光抗体检测显示 ,单抗不仅能识别恶性疟原虫子孢子 ,也能识别约氏疟原虫子孢子。 结论 成功地获得了针对恶性疟原虫CSP保守II+ 区的单克隆抗体。 |
| 关 键 词： | 疟原虫属 单克隆抗体 环子孢子蛋白 保守II+区 |
| 文章编号： | 1000-7423(2004)-02-0083-03 |
| 修稿时间： | 2003-06-11 |
Preparation and Identification of Monoclonal Antibodies against the Region II+ Motif in Circumsporozoite Protein of Plasmodium falciparum |
|
| ZHANG Rui-juan,ZHU Huai-min,LI Xiang-yu. Preparation and Identification of Monoclonal Antibodies against the Region II+ Motif in Circumsporozoite Protein of Plasmodium falciparum[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2004, 2(2): 83-85 | |
| Authors: | ZHANG Rui-juan ZHU Huai-min LI Xiang-yu |
| Affiliation: | Department of Etiological Biology, Second Military Medical University, Shanghai 200433, China. |
| Abstract: | OBJECTIVE: To develop and identify the monoclonal antibodies (McAbs) against Region II+ motif in circumsporozoite protein of Plasmodium falciparum. METHODS: BALB/c mice were immunized with 12 peptides within Region II+ in circumsporozoite protein of P. falciparum. Spleen cells isolated from the immunized mice were fused with myeloma cell. After three times screening with ELISA, 3 positive hybridoma cell lines were obtained. RESULTS: ELISA test indicated that the McAbs reacted with recombinant circumsporozoite protein fragment containing tandemly repeat region and conserved Region II+. IFA test showed that the McAbs recognized not only the sporozoites of P. falciparum, but also the sporozoifes of P. yoelii. CONCLUSION: McAbs obtained can probe the Region II+ motif in circumsporozoite protein of P. falciparum, which might also recognize that of other Plasmodium species. |
| Keywords: | Plasmodium')" >Plasmodium monoclonal antibody circumsporozoite protein +')" >Region II+ |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》浏览原始摘要信息 | |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》下载免费的PDF全文 | |