| 肥胖患者脂联素基因第45位点单核苷酸多态性与血浆脂联素水平及胰岛素抵抗的相关性研究 |
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| 引用本文： | 陈晓毓,李希圣,林夏鸿,高宏志,李秋兰,查金顺.肥胖患者脂联素基因第45位点单核苷酸多态性与血浆脂联素水平及胰岛素抵抗的相关性研究[J].国际放射医学核医学杂志,2012,36(5):269-273. |
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| 作者姓名： | 陈晓毓 李希圣 林夏鸿 高宏志 李秋兰 查金顺 |
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| 作者单位： | 1.362000 泉州，福建医科大学附属第二医院内分泌代谢科 |
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| 摘 要： | 目的 研究福建省泉州地区汉族肥胖人群脂联素基因第45位点单核苷酸多态性（SNP45）与血浆脂联素水平及胰岛素抵抗的关系。 方法 随机选择肥胖症患者248例，正常对照者223例，采用液相平衡竞争放射免疫分析法测定血浆中空腹胰岛素（FINS）水平；利用自动生化分析仪测定空腹血糖（FPG）、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）水平；计算体重指数（BMI）、腰臀比、稳态模型胰岛素抵抗指数（HOMA-IR）；采用酶联免疫吸附试验测定空腹血浆脂联素水平；采用PCR-限制性片段长度多态性技术检测脂联素基因SNP45。 结果 ① 肥胖症组和正常对照组脂联素基因SNP45的GT+GG基因型的分布分别为61%和44%（χ2=14.182，P＜0.01），G等位基因频率分别为35%和25%（χ2=10.708，P＜0.01）。②肥胖症组中，脂联素基因SNP45的GG+GT型与TT型相比，前者的TG、LDL-C水平（t=2.604, P＜0.01; t=5.507, P＜0.01）升高，而脂联素和HDL-C水平（t=2.275，P＜0.05；t=10.100，P＜0.01）降低。③正常对照组中，脂联素基因SNP45的GG+GT型与TT型相比，前者的脂联素、TG、TC水平（t=2.510，P＜0.05; t=2.922，P＜0.01; t=3.272，P＜0.01）显著低于后者。④以脂联素基因SNP45为因变量进行Logistic回归分析，肥胖症组中脂联素基因SNP45的GG+GT型与TT型相比，GG+GT型血清脂联素水平减少（OR=0.810，95%CI:0.673~0.975，P＜0.05），胰岛素抵抗风险增加（OR=1.746，95%CI:1.060~2.875，P＜0.05）；正常组中GG+GT型胰岛素抵抗风险增加（OR=3.962，95%CI:1.089~14.411，P＜0.05）。 结论 ① 脂联素基因SNP45的基因型和等位基因频率在肥胖症组与正常对照组中的分布有显著性差异。②脂联素基因SNP45与肥胖症患者的胰岛素抵抗及血脂水平相关。③肥胖症组中脂联素基因SNP45与脂联素水平相关。
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| 关 键 词： | 肥胖症 脂联素 多态性，单核苷酸 胰岛素抗药性 放射免疫测定 |
| 收稿时间： | 2012-05-24 |
Association of single nucleotide polymorphism at position 45 in adiponectin gene with plasma adiponectin level and insulin resistance in obesity |
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| Xiao-yu CHEN,Xi-sheng LI,Xia-hong LIN,Hong-zhi GAO,Qiu-lan LI,Jin-shun ZHA.Association of single nucleotide polymorphism at position 45 in adiponectin gene with plasma adiponectin level and insulin resistance in obesity[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(5):269-273. |
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| Authors: | Xiao-yu CHEN Xi-sheng LI Xia-hong LIN Hong-zhi GAO Qiu-lan LI Jin-shun ZHA |
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| Affiliation: | 1.Department of Endocrinology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China |
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| Abstract: | Objective To explore the association of single nucleotide polymorphism at position 45 （SNP45） in adiponectin gene with plasma adiponectin level and insulin resistance in obesity in Quanzhou area of Fujian province. Methods Two hundred and forty-eight patients with obesity and 225 normal con- trol subjects were enrolled in this study. Fasting insulin （FINS） were measured by radioimmunoassay and fasting plasma glucose （FPG）, total cholesterol （TC）, triglyeeride （TG）, high density lipoprotein-cholesterol （HDL-C）, low density lipoprotein-cholesterol （LDL-C） were measured by BECKMAN DXC800 biochemistry analyzer. Body mass index （BMI）, waist to hip ratio, homeostasis model assessment of insulin resistance （HOMA-IR） were calculated. Plasma adiponectin levels were examined by means of enzyme-linked immunosor- bentassy. The adiponectin gene SNP45 was identified by PCR-restriction fragment length polymorphism. Results ①Frequencies of GG＋GT genotype in obesity group and normal eontrol group were 61% and 44% respectively0（2=14.182, P〈0.01 ）, and G allele frequencies were 35% and 25%（X2=10.708, P〈0.01 ）. ②In obesity group, the subjects with SNP45 GG＋GT genotype had higher TG and LDL-C levels than those with TT genotype （t=2.604, P〈0.01; t=5.507, P〈0.01）, and had lower adiponectin level than those with TT genotype （t=2.275, P〈0.05）, and had signifieantly lower HDL-L level than those with TT genotype （t=10.100, P〈 0.01 ）. ③In normal eontrol group, the subjeets with SNP45 GG ＋GT genotype had signifieantly lower adiponeetin, TG, TC levels than those with TF genotype （t=2.510, P〈0.05; t=2.922, P〈0.01; t=3.272, P〈 0.01 ）. ④Logistie analysis proved that the SNP45 GG＋GT genotype in obesity group was associated with de- creased risk of plasma adiponeetin level （OR=0.810, 95%CI： 0.673-0.975, P〈0.05）, and with inereased risk of HOMA-IR （OR=1.746, 95%CI： 1.060-2.875, P〈0.05）. The SNP45 GG＋GT genotype in normal eon- trol group was associated with increased risk of HOMA-IR （OR =3.962, 95%CI： 1.089-14.411, P〈0.05）. Conclusions ⑤The distribution of adiponeetion of genotype and allele frequeneies of the SNP45 were dif- ferent in obesity and normal eontrol groups. ⑥The SNP45 in adiponeetin was assoeiated with obesity, insulin resistance and the total cholesterol, triglyeeride, high density lipoprotein, low density lipoprotein levels. ⑦The SNP45 in adiponectin was associated with the fasting adiponectin levels in obesity group. |
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| Keywords: | Obesity Adiponeetin Single nucleotide polymorphism Insulin resistance Radioim- munoassay |
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