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| 9-顺式维甲酸抑制PMA诱导人单核细胞系THP-1表达MMP-9 | |
| 引用本文： | 周磊,曾锦章,沈玲红,王力,胡刘华,何奔. 9-顺式维甲酸抑制PMA诱导人单核细胞系THP-1表达MMP-9[J]. 心脏杂志, 2007, 19(4): 373-376. DOI: 10.13191/j.chj.2007.04.7.zhoul.043 |
| 作者姓名： | 周磊 曾锦章 沈玲红 王力 胡刘华 何奔 |
| 作者单位： | 1. 上海交通大学医学院附属仁济医院心内科 |
| 基金项目： | 国家自然科学基金;上海市科委资助项目 |
| 摘 要： | 目的探讨视黄醛X受体（retinoid X receptors,RXRs）特异性激动剂9-顺式维甲酸（9-cisRA）对佛波酯（PMA）诱导人单核细胞系THP-1基质金属蛋白酶-9（MMP-9）表达及活性的影响。方法体外培养THP-1细胞,PMA诱导分化为巨噬细胞,采用9-cisRA对不同浓度PMA组进行干预,应用Realtime-PCR、Westerblotting测定THP-1细胞MMP-9的基因和蛋白水平表达水平,通过Gelatin Zymography法检测MMP-9的酶活性。结果9-cisRA（100nmol/L）对不同浓度PMA组（10、20和40 nmol/L）干预24 h,9-cisRA可明显抑制THP-1细胞MMP-9转录水平,MMP-9的mRNA抑制率分别28%、60%、88%（P<0.01）。MMP-9蛋白水平及酶活性也呈显著下降。结论RXRs特异性激动剂9-cisRA可显著抑制PMA诱导THP-1的MMP-9转录和蛋白水平表达及其酶活性。 |
| 关 键 词： | 9-顺式维甲酸 视黄醛X受体 基质金属蛋白酶-9 动脉粥样硬化 |
| 文章编号： | 1009-7236（2007）04-373-04 |
| 收稿时间： | 2007-01-29 |
| 修稿时间： | 2007-03-16 |
9-cisRA inhibits expression of MMP-9 in THP-1 human monocytes induced by PMA |
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| ZHOU Lei,ZENG Jin-zhang,SHEN Ling-hong,WANG Li,HU Liu-hua,HE Ben. 9-cisRA inhibits expression of MMP-9 in THP-1 human monocytes induced by PMA[J]. Chinese Heart Journal, 2007, 19(4): 373-376. DOI: 10.13191/j.chj.2007.04.7.zhoul.043 | |
| Authors: | ZHOU Lei ZENG Jin-zhang SHEN Ling-hong WANG Li HU Liu-hua HE Ben |
| Affiliation: | 1. Department of Cardiology, Renji Hospital, Shanghai Jiaotong University, Shanghai 200001, China; 2. Shanghai Institutes for Biological Sciences, Shanghai 200233, China |
| Abstract: | AIM To investigate the effect of 9-cisRA(9-cis-Retinoic Acid),a specific agonist of retinoid X receptors(RXRs),on the expression and activities of matrix metalloproteinase-9(MMP-9) in THP-1 human monocytes induced by PMA.METHODS Human monocytic cell lines THP-1 were cultured and induced to macrophages by PMA treatment.The differentiated monocytes were then cultured in the medium of PMA at different concentrations treated with 9-cisRA(100 nmol/L) and the gene expression,protein expression and activity of MMP-9 were detected by Realtime-PCR,Western blotting and Gelatin Zymography respectively.RESULTS After 24 h treatment of 100 nmol/L 9-cisRA,the mRNA level of MMP-9 in THP-1 cells induced by PMA at different concentrations(10,20 and 40 nmol/L) decreased by 28%,60%,88%(P<0.01) respectively,while the protein level and activity of MMP-9 also dropped significantly.CONCLUSION 9-cisRA,a specific agonist of RXRs,effectively inhibits the mRNA,protein level and activity of MMP-9 in THP-1 cells induced by PMA. |
| Keywords: | 9-cis-Retinoic Acid retinoid X receptors matrix metalloproteinase-9 atherosclerosis |
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