| 柏氏禽刺螨传播家鼠型汉坦病毒的研究 |
| |
| 引用本文： | 诸葛洪祥,孟阳春,吴建伟,朱智勇,梁伟峰,姚萍萍.柏氏禽刺螨传播家鼠型汉坦病毒的研究[J].中国寄生虫学与寄生虫病杂志,1998,16(6):445-448. |
| |
| 作者姓名： | 诸葛洪祥 孟阳春 吴建伟 朱智勇 梁伟峰 姚萍萍 |
| |
| 作者单位： | 苏州医学院寄生虫学教研室，浙江省卫生防疫站 |
| |
| 摘 要： | 目的：研究柏氏禽刺螨在传播家鼠型肾综合征出血热病毒中的作用。方法：每罐放柏氏禽刺螨100只，于室温为23±1℃环境下饥饿4d，分别叮刺感染汉坦病毒（Hantaanvirus）或浙45株（Z45）大鼠乳小鼠2只或感染汉城病毒（Seoulvirus）UR株大鼠乳小鼠2只各12h，为感染螨，用感染后d15的螨叮刺正常大鼠乳小鼠9只，d30取感染鼠的肺和血清，用间接免疫荧光测试。于d15和d23取感染螨用聚合酶链反应测试其体内病毒RNA。结果：柏氏禽刺螨分别叮刺感染汉坦病毒Z45株和感染汉城病毒UR株大鼠乳小鼠后, 再分别叮刺正常大鼠乳小鼠5 只和4 只。IFAT 检测结果汉坦病毒Z45 株组的鼠全部阳性, 汉城病毒UR 株组的4 只鼠中3 只阳性, 其抗体滴度, 前者为10- 40, 后者为20- 40, 病毒在螨体内可保存22 d 以上。阻断试验显示, 病毒免疫血清1∶20 作用肺切片标本后, 部分阻断荧光抗体反应, 与未经稀释的血清作用后全部阻断特异性荧光反应。对照组正常鼠肺片和血清经IFA T 检测均阴性。结论: 柏氏禽刺螨可作为家鼠型肾综合征出血热的传播媒介和贮存宿主。
|
| 关 键 词： | 柏氏禽刺螨 家鼠型汉坦病毒 叮刺传播 |
STUDIES ON THE EXPERIMENTAL TRANSMISSION OF RATTUS BORNE HANTAVIRUS BY ORNITHONYSSUS BACOTI |
| |
| Zhuge Hongxiang,Meng Yangchun,Wu Janwei,Zhu Zhiyong,Liang Wiefeng,Yao Pingping.STUDIES ON THE EXPERIMENTAL TRANSMISSION OF RATTUS BORNE HANTAVIRUS BY ORNITHONYSSUS BACOTI[J].Chinese Journal of Parasitology and Parasitic Diseases,1998,16(6):445-448. |
| |
| Authors: | Zhuge Hongxiang Meng Yangchun Wu Janwei Zhu Zhiyong Liang Wiefeng Yao Pingping |
| |
| Affiliation: | Department of Parasitology, Suzhou Medical College, Suzhou 215007. |
| |
| Abstract: | AIM: To demonstrate the role of rat mite (Ornithonyssus bacoti) in the transmission of Rattus-borne hemorrhagic fever with renal syndrome (HFRS). METHODS: In the transmission experiments, about 100 O. bacoti per pool were isolated and placed in a jar, unfed for 4 d at 23 +/- 1 degrees C. Suckling Wistar rats inoculated with Hantavirus strain Z45 or Seoul virus strain UR were placed in each jar for free attack by the mites for 12 hours. After 14 d the normal suckling Wistar rats were bitten by the mites. Fifteen days later, the lung tissues and sera of the infected rats were collected and detected for Hantaviral antigen by indirect fluorescent antibody technique (IFAT). For demonstration of the infection of O. bacti with Rattus-borne Hantavirus PCR technique was applied to detect Rattus-borne Hantaviral RNA. RESULTS: Sukling Wistar rats inoculted Hantavirus strain Z45 or Seoul virus strain UR were bitten by O. bacoti and then these mites were fed on 4 and 5 of normal suckling rats in each jar, respectively. The antigens of Hantavirus strain Z45 were positive in all the lungs of the normal rats bitten by the mites, the sera titers of the rats were from 1:10 to 1:40. The antigens were positive in 3 of the 4 rats, the sera titers were from 1:20 to 1:40. Both of the viruses could be maintained in O. bacoti for 22 days. The blocking test showed when 1:30 Hantavirus immunosera were exposed to the lung samples and then reacted with the sera from the patients with HFRS, all the specific fluorescence reactions of the samples were blocked, whereas the control group including the normal rat lung tissues and sera were all negative (Fig. 1). CONCLUSION: O. bacoti might play a role as the vector of HFRS and a reservoir host as well. |
| |
| Keywords: | Ornithonyssus bacoti Rattus borne hantavirus biting transmission |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》浏览原始摘要信息 |
|
点击此处可从《中国寄生虫学与寄生虫病杂志》下载全文 |
|
