| miR-127对皮肤鳞状细胞癌细胞生物学行为调控作用研究 |
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| 引用本文： | 李滋泽,岳振,胡楠.miR-127对皮肤鳞状细胞癌细胞生物学行为调控作用研究[J].蚌埠医学院学报,2022,47(8):999-1002. |
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| 作者姓名： | 李滋泽 岳振 胡楠 |
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| 作者单位： | 1.蚌埠医学院 临床医学院, 安徽 蚌埠 2330302.蚌埠医学院第一附属医院 皮肤科, 安徽 蚌埠 2330043.南京鼓楼医院 血管外科, 江苏 南京 210008 |
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| 摘 要： | 目的探讨miR-127对皮肤鳞状细胞癌(鳞癌)细胞的生物学行为调控作用。方法收集皮肤鳞癌组织、癌旁正常组织标本，采用实时定量PCR检测miR-127在2组标本中的表达水平。调控鳞癌细胞株A431中miR-127表达水平，检测鳞癌细胞增殖、侵袭能力的变化。通过生物信息学预测网站及分子生物学方法验证miR-127的作用靶点。结果miR-127在皮肤鳞癌组织中表达明显高于癌旁组织(P < 0.01)。相较于HaCaT细胞，miR-127表达水平在A431细胞以及HSC-5细胞中显著升高，差异有统计学意义(P < 0.05)。上调miR-127表达后，A431细胞的增殖及侵袭能力显著增强；下调miR-127的表达后，A431细胞的增殖及侵袭能力受到抑制(P < 0.05)。通过双荧光素酶报告基因实验发现miR-127可以与PTEN直接结合，调控miR-127后PTEN在蛋白水平的表达发生改变(P < 0.05)。结论miR-127参与调控鳞癌细胞的生物学行为，miR-127可能作为治疗皮肤鳞癌的一个潜在的靶点。
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| 关 键 词： | 皮肤鳞状细胞癌 miR-127 增殖 侵袭 |
| 收稿时间： | 2022-03-31 |
Study on the regulatory effects of miR-127 on biological behavior of cutaneous quamous cell carcinoma cells |
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| Affiliation: | 1.School of Clinical, Bengbu Medical College, Bengbu Anhui 2330302.Department of Dermatology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 2330043.Department of Surgery, Nanjing Drum Tower Hospital, Nanjing Jiangsu 210008, China |
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| Abstract: | ObjectiveTo investigate the regulatory effects of miR-127 on biological behavior of skin squamous cell carcinoma cells.MethodsThe samples of skin squamous cell carcinoma tissues and adjacent normal tissues were collected, and the expression levels of miR-127 in two groups were detected by real-time quantitative PCR.The expression level of miR-127 in squamous cell carcinoma cell line A431 was regulated to detect the changes of proliferation and invasion ability of squamous cell carcinoma cells.The targets of miR-127 were verified by bioinformatics prediction websites and molecular biological methods.ResultsThe expression level of miR-127 in skin squamous cell carcinoma tissues was significantly higher than that in adjacent tissues(P < 0.01).Compared with HaCaT cells, the expression levels of miR-127 in A431 cells and HSC-5 cells significantly increased, and the difference of which was statistically significant(P < 0.05).After up-regulating the expression of miR-127, the proliferation and invasion ability of A431 cells were significantly enhanced(P < 0.05).The proliferation and invasion ability of A431 cells were inhibited after down-regulating the expression of miR-127(P < 0.05).The results of dual luciferase reporter gene assay showed that the miR-127 could directly bind to PTEN, and the expression level of PTEN proetin was changed after regulating miR-127(P < 0.05).ConclusionsThe miR-127 is involved in the regulation of biological behavior of squamous cell carcinoma cells.miR-127 may be used as a potential molecular target in the treatment of skin squamous cell carcinoma. |
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