| 敲低galectin-1可抑制肺腺癌细胞的增殖、迁移和侵袭并促进其凋亡 |
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| 引用本文： | 陈文邦,朱 潇,周 少,杏福宝,唐 震,李小军,张 雷. 敲低galectin-1可抑制肺腺癌细胞的增殖、迁移和侵袭并促进其凋亡[J]. 南方医科大学学报, 2022, 42(11): 1628-1637. DOI: 10.12122/j.issn.1673-4254.2022.11.06 |
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| 作者姓名： | 陈文邦 朱 潇 周 少 杏福宝 唐 震 李小军 张 雷 |
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| 作者单位： | 蚌埠医学院癌症转化医学安徽省重点实验室，第一附属医院胸外科，安徽 蚌埠 233040 |
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| 摘 要： | 目的 探讨半乳糖凝集素-1（galectin-1）对肺腺癌细胞的影响及其机制。方法 收集并比较肺腺癌组织和癌旁正常组织galectin-1的表达水平。qRT-PCR法检测肺腺癌细胞系A549、H1299与正常支气管上皮细胞细胞BEAS-2b中galectin-1的表达差异。通过si-RNA敲低galectin-1，分为对照组和si-RNA组，对照组转染同剂量NC-siRNA片段。通过qRT-PCR和Westernblot检测galectin-1mRNA和蛋白水平的表达情况。CCK8、Transwell实验、划痕实验和流式细胞术检测敲低galectin-1后对肺腺癌细胞增殖能力、侵袭和迁移能力和细胞凋亡的情况。Western blot检测敲低galectin-1后凋亡相关蛋白BAX、BCL-2、Caspase3等蛋白和AKT、ERK二条通路蛋白的相对表达情况。结果 galectin-1的mRNA在肺癌组织和肺腺癌细胞株中表达量明显升高（P<0.05）。抑制galectin-1表达后，细胞增殖、迁移和侵袭等下降，凋亡率明显升高（P<0.05）。在抑制galectin-1表达后,ERK信号通路磷酸化水平明显降低（P<0.05）。结论 galectin-1具有抑制肺腺癌细胞增殖、迁移、侵袭和促进其凋亡的作用，其机制可能与ERK通路的磷酸化激活水平有关。
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| 关 键 词： | 肺腺癌；galectin-1；增殖；侵袭；迁移；凋亡；ERK信号通路 |
Galectin-1 knockdown inhibits proliferation,migration, invasion and promotes apoptosis of lung adenocarcinoma cells in vitro |
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| CHEN Wenbang,ZHU Xiao,ZHOU Sao,XING Fubao,TANG Zhen,LI Xiaojun,ZHANG Lei. Galectin-1 knockdown inhibits proliferation,migration, invasion and promotes apoptosis of lung adenocarcinoma cells in vitro[J]. Journal of Southern Medical University, 2022, 42(11): 1628-1637. DOI: 10.12122/j.issn.1673-4254.2022.11.06 |
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| Authors: | CHEN Wenbang ZHU Xiao ZHOU Sao XING Fubao TANG Zhen LI Xiaojun ZHANG Lei |
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| Affiliation: | Anhui Provincial Key Laboratory of Translational Cancer Medicine, Department of Thoracic Surgery, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China |
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| Abstract: | Objective To investigate the effect of galectin-1 on biological behaviors of lung adenocarcinoma cells and the underlying mechanism. Methods The expression levels of galectin-1 mRNA were detected in 8 pairs of lung adenocarcinoma tissues and adjacent normal tissues and in lung adenocarcinoma cell lines A549 and H1299 and normal bronchial epithelial cell line BEAS-2b using qRT-PCR. The effect of galectin-1 knockdown by RNA interference on the proliferation, invasion and migration abilities and apoptosis of lung adenocarcinoma cells were examined using CCK8 assay, Transwell assay, scratch assay and flow cytometry. Western blotting was performed to detect the expressions of apoptosis-related proteins BAX, BCL-2, and caspase-3 and the proteins involved in the AKT and ERK pathways. Results The mRNA expression of galectin-1 was significantly increased in lung cancer tissues and lung adenocarcinoma cell lines (P<0.05). In lung adenocarcinoma cells, galectin-1 knockdown significantly inhibited cell proliferation, migration and invasion and obviously increased cell apoptosis rate (P<0.05), causing also significant reduction of the phosphorylation level of ERK signaling pathway (P<0.05). Conclusion Galectin-1 knockdown inhibits proliferation, migration, and invasion and promotes apoptosis of lung adenocarcinoma cells, and this effect is mediated probably by inhibition of the phosphorylation levels of the ERK pathway. |
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| Keywords: | lung adenocarcinoma galectin-1 proliferation invasion migration apoptosis ERK signaling pathway, |
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