| 蝎毒耐热蛋白对癫痫大鼠海马组织BDNF及NPY表达的影响 |
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| 引用本文： | 陈其钻,杨朋范,钟忠辉,林,巧,梅,珍,裴家生,王守森.蝎毒耐热蛋白对癫痫大鼠海马组织BDNF及NPY表达的影响[J].中国临床神经外科杂志,2020,0(2):98-100. |
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| 作者姓名： | 陈其钻 杨朋范 钟忠辉 林 巧 梅 珍 裴家生 王守森 |
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| 作者单位： | 350025 福州，中国人民解放军联勤保障部队第900医院神经外科（陈其钻、杨朋范、钟忠辉、林 巧、梅、珍、裴家生、王守森） |
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| 摘 要： | 目的探讨蝎毒耐热蛋白（SVHRP）对癫痫大鼠海马组织脑源性神经营养因子（BDNF）和神经肽Y（NPY）表达的影响。方法 健康成年雄性SD大鼠180只，按随机区组法随机分为3组（SVHRP组、模型组、正常组），每组60只动物。经颈部皮下注射FeCl2诱发癫痫急性发作，选取4~5级（Racine的癫痫分级标准）癫痫发作的大鼠。HE染色观察大鼠海马组织形态，免疫印迹法及荧光免疫组织化学染色检测大鼠海马组织BDNF和NPY的表达。结果 HE染色结果显示模型组大鼠海马组织形态结果破坏严重，而SVHRP组明显改善。模型组和SVHRP组大鼠海马神经BDNF及NPY表达水平均明显低于正常组（P<0.05），但SVHRP组BDNF及NPY 表达水平明显高于模型组（P<0.05）。结论 SVHRP能够减轻癫痫大鼠海马组织病理损伤程度，上调海马组织BDNF及NPY表达。
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| 关 键 词： | 癫痫 蝎毒耐热蛋白 FeCl2诱导癫痫模型 大鼠 |
Effects of scorpion venom heat-resistant protein on expressions of BDNF and NPY in hippocampal tissues in rats with epilepsy |
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| CHEN Qi-zuan,YANG Peng-fan,ZHONG Zhong-hui,LIN Qiao,MEI Zhen,PEI Jia-shen,WANG Shou-sen..Effects of scorpion venom heat-resistant protein on expressions of BDNF and NPY in hippocampal tissues in rats with epilepsy[J].Chinese Journal of Clinical Neurosurgery,2020,0(2):98-100. |
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| Authors: | CHEN Qi-zuan YANG Peng-fan ZHONG Zhong-hui LIN Qiao MEI Zhen PEI Jia-shen WANG Shou-sen |
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| Affiliation: | Department of Neurosurgery, No. 900 Hospital, Logistic Support, Command, PLA, Fuzhou 350025, China |
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| Abstract: | Objective To investigate the effect of scorpion venom heat-resistant protein (SVHRP) on the expressions of BDNF and NPY in hippocampal tissues in rats with epilepsy. Methods One hundred and eighty rats were randomly divided into 3 groups of 60 animals each, i.e. SVHRP treatment group, model group and normal group. The epileptic models were made by subcutaneous injection of FeCl2. The expressions of brain-derived neurotrophic factor (BDNF) and neuropeptide Y (NPY) in the hippocampal tissues was detected by western blot and fluorescence immunohistochemical technique in all the animals. Results The levels of expressions of BDNF and NPY in hippocampal tissues were significantly higher in the normal group than those in the SVHRP treatment group (P<0.05), which were significantly higher than those in the model group (P<0.05). Conclusions SVHRP can increase the expressions of BDNF and NPY expressions in the hippocampal tissues in the rats with epilepsy. |
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| Keywords: | Scorpion venom heat-resistant protein FeCl2 Epilepsy Hippocampal neurons BDNF NPY Rats |
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