Usefulness of optical coherence tomography to detect central serous chorioretinopathy in monkeys |
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| Authors: | Hyun‐Kyu Park Woori Jo Hyun‐Ji Choi Bongcheol Kim Gilnam Lee Jeongbeob Seo Suk Young Cho Choung‐Soo Kim Eun Kyung Choi Jung Jin Hwang Joo Yong Lee Young Hee Yoon Woo‐Chan Son |
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| Affiliation: | 1. Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea;2. Institute for Innovative Cancer Research, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea;3. Department of Medicinal Chemistry, CHABIOMED Co. LTD, Seongnam‐Si, Korea;4. Chemistry Service, WuXi App Tec Co. LTD, Shanghai, China;5. Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea;6. Department of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea;7. Department of Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea;8. Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea |
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| Abstract: | Many systemic drugs can induce ocular toxicity and several ocular side‐effects have been identified in clinical studies. However, it is difficult to detect ocular toxicity in preclinical studies because of the lack of appropriate evaluation methods. Optical coherence tomography (OCT) is useful because it can provide real‐time images throughout a study period, whereas histopathology only provides images of sacrificed animals. Using OCT alongside histopathology, attempts were made to find effective approaches for screening of drug‐induced ocular toxicity in monkeys. Such approaches could be used in preclinical studies prior to human trials. Six male cynomolgus monkeys (Macaca fascicularis Raffles) were orally administered one of six candidate MAPK/ERK kinase (MEK) inhibitors. Central serous chorioretinopathy, a known side‐effect of such inhibitors, was identified in four monkeys by OCT. Artifacts generated during tissue processing meant that histopathology could not detect edematous changes. Thus, OCT is a useful tool to detect ocular toxicity which cannot be detected by histopathology in preclinical studies. Copyright © 2014 John Wiley & Sons, Ltd. |
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| Keywords: | ocular toxicity central serous chorioretinopathy optical coherence tomography monkey MEK inhibitor |
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