Aluminium oxide nanoparticles induced morphological changes,cytotoxicity and oxidative stress in Chinook salmon (CHSE‐214) cells |
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| Authors: | Koigoora Srikanth Amit Mahajan Eduarda Pereira Armando Costa Duarte Janapala Venkateswara Rao |
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| Affiliation: | 1. CESAM‐Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, Aveiro, Portugal;2. Department of Bioresource Sciences, Andong National University, Andong, Republic of Korea;3. Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad, India;4. Department of Materials and Ceramics Engineering, CIECO, University of Aveiro, Aveiro, Portugal |
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| Abstract: | Aluminium oxide nanoparticles (Al2O3 NPs) are increasingly used in diverse applications that has raised concern about their safety. Recent studies suggested that Al2O3 NPs induced oxidative stress may be the cause of toxicity in algae, Ceriodaphnia dubia, Caenorhabditis elegans and Danio rerio. However, there is paucity on the toxicity of Al2O3 NPs on fish cell lines. The current study was aimed to investigate Al2O3 NPs induced cytotoxicity, oxidative stress and morphological abnormality of Chinnok salmon cells (CHSE‐214). A dose‐dependent decline in cell viability was observed in CHSE‐214 cells exposed to Al2O3 NPs. Oxidative stress induced by Al2O3 NPs in CHSE‐214 cells has resulted in the significant reduction of superoxide dismutase, catalase and glutathione in a dose‐dependent manner. However, a significant increase in glutathione sulfo‐transferase and lipid peroxidation was observed in CHSE‐214 cells exposed to Al2O3 NPs in a dose‐dependent manner. Significant morphological changes in CHSE‐214 cells were observed when exposed to Al2O3 NPs at 6, 12 and 24 h. The cells started to detach and appear spherical at 6 h followed by loss of cellular contents resulting in the shrinking of the cells. At 24 h, the cells started to disintegrate and resulted in cell death. Our data demonstrate that Al2O3 NPs induce cytotoxicity and oxidative stress in a dose‐dependent manner in CHSE‐214 cells. Thus, our current work may serve as a base‐line study for future evaluation of toxicity studies using CHSE‐214 cells. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | aluminium oxide nanoparticles Chinook salmon cells cytotoxicity oxidative stress morphological abnormality |
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