Z-DNA-forming sites within the human beta-globin gene cluster. |
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| Authors: | V Müller M Takeya S Brendel B Wittig A Rich |
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| Affiliation: | Abteilung Molekularbiologie und Bioinformatik, Freie Universität Berlin, Germany. |
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| Abstract: | ![]()
Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, and five were found in the Z conformation. |
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