Identification of Histoplasma capsulatum from culture extracts by real-time PCR |
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Authors: | Martagon-Villamil Jose Shrestha Nabin Sholtis Mary Isada Carlos M Hall Gerri S Bryne Terry Lodge Barbara A Reller L Barth Procop Gary W |
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Affiliation: | Division of Infectious Diseases. Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA. |
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Abstract: | We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism. |
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