| 过量氟对大鼠切牙基质金属蛋白酶-20及其组织抑制剂表达的影响 |
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| 引用本文： | 井枫秋,王强,刘天麟,郭丽英,刘辉.过量氟对大鼠切牙基质金属蛋白酶-20及其组织抑制剂表达的影响[J].华西口腔医学杂志,2006,24(3):199-201. |
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| 作者姓名： | 井枫秋 王强 刘天麟 郭丽英 刘辉 |
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| 作者单位： | 同济大学附属口腔医院 口腔内科，上海 200072 |
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| 基金项目： | 同济大学校科研和教改项目 |
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| 摘 要： | 目的 研究过量氟对大鼠切牙生长过程中基质金属蛋白酶-20（MMP-20）和基质金属蛋白酶组织抑制剂-2（TIMP-2）表达的影响，探讨氟斑牙的发病机制。方法 选择20只Wistar大鼠，随机分为对照组和给氟组。8 周后处死动物，获取切牙标本，免疫组化染色观察MMP-20和TIMP-2在大鼠切牙中的表达定位和氟对二者表达的影响。结果 MMP-20和TIMP-2 在分泌期成釉细胞、成牙本质细胞以及成釉器均有阳性表达，给氟组MMP-20的表达明显弱于对照组，差异有统计学意义（P<0.01）；TIMP-2在两组的表达无显著性差异（P>0.05）。结论 过量氟可抑制MMP-20的表达, MMP-20和TIMP-2表达失衡，TIMP-2的抑制作用加强，致使釉基质蛋白清除延迟，导致矿化不全和釉质缺损。
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| 关 键 词： | 氟 大鼠切牙 基质金属蛋白酶 基质金属蛋白酶组织抑制剂 |
| 文章编号： | 1000-1182（2006）03-0199-03 |
| 收稿时间： | 2006-06-25 |
| 修稿时间： | 2005年11月30 |
Effects of Overdosed Fluoride on Rat's Incisor Expression of Matrixmetalloproteinase-20 and Tissue Inhibitors of Metalloproteinase-2 |
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| JING Feng-qiu,WANG Qiang,LIU Tian-lin,GUO Li-ying,LIU Hui.Effects of Overdosed Fluoride on Rat''''s Incisor Expression of Matrixmetalloproteinase-20 and Tissue Inhibitors of Metalloproteinase-2[J].West China Journal of Stomatology,2006,24(3):199-201. |
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| Authors: | JING Feng-qiu WANG Qiang LIU Tian-lin GUO Li-ying LIU Hui |
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| Affiliation: | Dept. of Oral Medicine，Hospital of Stomatology, Tongji University, Shanghai 200072, China |
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| Abstract: | Objective To investigate the effects of overdosed fluoride on the expression of MMP-20 and TIMP-2 in rat incisor.Methods 20 Wistar rats were randomly divided into experiment group and control group.The distilled water was given in control group.100 mg/L fluoride-was given in experiment group.After 8 weeks treatment,the rats were killed.Immunohistochemical staining was used to observe the expression of MMP-20 and TIMP-2 in rat incisors.Results Immunohistochemical results demonstrated the presence of MMP-20 and TIMP-2 protein in ameloblasts,odontoblasts,stratum intermedium and the stellate reticulum of rat incisor.The imagination analysis results showed that the expression of MMP-20 was reduesed in experiment group(P<0.01),and the expression of TIMP-2 had no significant difference(P>0.05).Conclusion The overdosed fluoride inhibits the secretion of MMP-20 and leads to the disturbed balance between MMP-20,TIMP-2 in rat incisor,which leads to the delay of the amelogenin removal and the enamel demineralization. |
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| Keywords: | fluoride rat incisor matrix metailoproteinase-20 tissue inhibitors of metalloproteinase-2 |
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