The influence of humic acid on the toxicity of nano‐ZnO and Zn2+ to the Anabaena sp. |
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| Authors: | Yulin Tang Shuyan Li Yao Lu Qian Li Shuili Yu |
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| Affiliation: | State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, People's Republic of China |
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| Abstract: | This study explored the effects of humic acid (HA) on the toxicity of ZnO nanoparticles (nano‐ZnO) and Zn2+ to Anabaena sp. Typical chlorophyll fluorescence parameters, including effective quantum yield, photosynthetic efficiency and maximal electron transport rate, were measured by a pulse‐amplitude modulated fluorometer. Results showed that nano‐ZnO and Zn2+ could inhibit Anabaena sp. growth with the EC50 (concentration for 50% of maximal effect) of 0.74 ± 0.01 and 0.3 ± 0.01 mg/L, respectively. In the presence of 3.0 mg/L of HA, EC50 of nano‐ZnO increased to 1.15 ± 0.04 mg/L and EC50 of Zn2+ was still 0.3 ± 0.01 mg/L. Scanning electron microscopy observation revealed that HA prevented the adhesion of nano‐ZnO on the algae cells due to the increased electrostatic repulsion. The generation of intracellular reactive oxygen species and cellular lipid peroxidation were significantly limited by HA. Nano‐ZnO had more damage to the cell membrane than Zn2+ did, which could be proven by the malondialdehyde content in Anabaena sp. cells. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 895–903, 2015. |
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| Keywords: | natural organic matter algae toxicity ZnO nanoparticles zinc |
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