Localization of two epitopes recognized by monoclonal antibody PCG-4 on Clostridium difficile toxin A. |
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| Authors: | S M Frey and T D Wilkins |
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| Affiliation: | Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061. |
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| Abstract: | The toxin A gene of Clostridium difficile contains a 2.5-kb region encoding a series of contiguous repeating units located at the COOH terminus of the molecule. We previously showed that the monoclonal antibody (MAb) PCG-4, which neutralizes the enterotoxic activity of toxin A, binds to epitopes located within these repeating units. In the present study, we subcloned a series of fragments from this portion of the gene. The recombinant peptides expressed from the gene fragments were examined for reactivity with MAb PCG-4 to identify the epitopes involved in binding. Our results showed that MAb PCG-4 recognizes epitopes in amino acid residues 2097 through 2141 and amino acid residues 2355 through 2398. |
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