Release of transforming growth factor-beta (TGF-β) and fibronectin by alveolar macrophages in airway diseases |
| |
| Authors: | A M VIGNOLA P CHANEZ G CHIAPPARA A MERENDINO E ZINNANTI J BOUSQUET V BELLIA G BONSIGNORE |
| |
| Affiliation: | *Istituto di Fisiopatologia Respiratoria, C.N.R., Palermo, Italy;†Clinique des Maladies Respiratoires and CJF 92-10 INSERM, Hôpital Arnaud de Villeneuve, Montpellier, France;‡Clinica di Malatie Respiratorie, Universita di Palermo, Palermo, Italy |
| |
| Abstract: | ![]()
Asthma and chronic bronchitis are associated with airway remodelling, and airway macrophages are present in bronchial inflammation. TGF-β and fibronectin released by alveolar macrophages possess a fibrogenic potency. The potential role of alveolar macrophages in airway remodelling was studied in asthma and chronic bronchitis by the release of TGF-β and fibronectin. Alveolar macrophages were isolated by bronchoalveolar lavage in 14 control subjects, 14 asthmatics and 14 chronic bronchitics. The spontaneous and lipopolysaccharide (LPS)- or concanavalin A (Con A)-induced release of TGF-β and fibronectin was measured by ELISA. Alveolar macrophages from chronic bronchitics spontaneously release greater amounts of TGF-β and fibronectin than those from asthmatic and control subjects. Alveolar macrophages from asthmatics release greater amounts of TGF-β and fibronectin than those from control subjects. The spontaneous release of TGF-β is significantly correlated with that of fibronectin. Fibronectin release was significantly reduced after LPS stimulation, and TGF-β release was significantly increased after LPS stimulation, except in chronic bronchitis patients. Con A increased the release of TGF-β in cells from normal subjects. This study suggests that activated macrophages play a role in airway remodelling in chronic bronchitis and to a lesser extent in asthma. |
| |
| Keywords: | remodelling transforming growth factor-beta fibronectin asthma chronic bronchitis alveolar macrophages |
|
|
