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| 骨缝牵引中联合应用骨形态发生蛋白-2和骨保护素的实验研究 | |
| 引用本文： | Yao Y,Huang H,Chang S,Wang C,Wang G. 骨缝牵引中联合应用骨形态发生蛋白-2和骨保护素的实验研究[J]. 华西口腔医学杂志, 2012, 30(4): 425-429. DOI: 10.3969/j.issn.1000-1182.2012.04.024 |
| 作者姓名： | Yao Y Huang H Chang S Wang C Wang G |
| 作者单位： | 1.辽宁医学院附属第二医院口腔颌面外科, 锦州121000; 2.北京友谊医院口腔颌面外科, 北京100050;3.辽宁医学院药学系, 锦州121000 |
| 基金项目： | 辽宁省教育厅基金资助项目(L2010281) |
| 摘 要： | 目的探索上颌骨在骨缝牵引时加入缓释骨形态发生蛋白-2（BMP-2）和骨保护素（OPG）对新骨形成的影响。方法以24只杂种犬为研究对象,随机分为A、B、C组。通过手术在上颌骨腭横缝植入自制的新型牵引器。A、C组术后5 d在牵引区附近注射缓释重组人骨形态发生蛋白-2/聚乳酸-羟基乙酸共聚物/纤维蛋白胶（rhBMP-2/PLGA/FS）,B、C组在牵引3周后注射人骨保护素/纤维蛋白胶（rhOPG/FS）。牵引1、2、4、6周后处死动物,采集标本进行组织学染色,并通过组织计量学方法检测腭横缝的组织改建情况。结果A、C组骨缝区成骨细胞功能活跃,透射电镜显示细胞内有大量高尔基复合体、线粒体及粗面内质网。牵引6周时,A、B、C组成骨细胞指数分别为38.5±7.7、35.7±6.5、41.7±11.0,破骨细胞指数分别为5.9±1.0、1.2±0.3、2.8±0.4,骨小梁厚度分别为（38.36±13.28）、（66.20±9.16）、（51.85±9.92）μm;B、C组表现出骨密度增加及破骨细胞指数下降。结论本实验所用牵引器能促进新骨生成;BMP-2与OPG在骨缝牵引过程中有协同作用,可以促进新骨形成及骨改建。 |
| 关 键 词： | 牵引成骨术 骨形态发生蛋白-2 骨保护素 |
| 收稿时间： | 2011-03-14 |
| 修稿时间： | 2011-11-05 |
The effect of bone morphogenetic protein-2 and osteoprotegerin in trans-sutural distraction osteogenesis |
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| Yao Yusheng,Huang Hua,Chang Shimin,Wang Chengyue,Wang Guijun. The effect of bone morphogenetic protein-2 and osteoprotegerin in trans-sutural distraction osteogenesis[J]. West China journal of stomatology, 2012, 30(4): 425-429. DOI: 10.3969/j.issn.1000-1182.2012.04.024 | |
| Authors: | Yao Yusheng Huang Hua Chang Shimin Wang Chengyue Wang Guijun |
| Affiliation: | 1. Dept. of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, China; 2. Dept. of Oral and Maxillofacial Surgery, Beijing Friendship Hospital, Beijing 100050, China; 3. Pharmacy Department, Liaoning Medical College, Jinzhou 121000, China |
| Abstract: | Objective To determine if locally administered bone morphogenetic protein-2(BMP-2) and osteoprotegerin(OPG) improved osteogenesis and new bone formation by trans-sutural distraction osteogenesis.Methods Twenty four dogs were divided into three groups randomly and received new internal trans-sutural distraction osteogenesis treat-ment.Five days after operation,infusion apparatus with double-tube was inserted to submucosa near the distracted zone to deliver controlled release agent of recombinant human bone morphogenetic protein-2/poly(lactic-co-glycolic acid)/ fibrin sealant(rhBMP-2/PLGA/FS) in group A and group C.Recombinant human osteoprotegerin/fibrin sealant(rhOPG/ FS) was injected three weeks later in group B and group C.Histology staining and bone histomorphometry were used to measure the changes of maxillary bone sutura after distraction for 1,2,4 and 6 weeks.Results New bone formation observed in distracted zone showed a significant increase in group A and C.Transmission electron microscope showed the osteoblast and osteocyte were active with dilated rough endoplasmic reticulum and a large number of chondriosomes and Golgi complex.After distraction for 6 weeks,indexes of osteoblast of group A,B,and C were 38.5±7.7,35.7±6.5,and 41.7±11.0,indexes of osteoclast(Ioc) were 5.9±1.0,1.2±0.3,and 2.8±0.4,bone trabecula thicknesses were(38.36± 13.28),(66.20±9.16),and(51.85±9.92) μm respectively.Increased bone density and decreased Ioc were found in group B and C.Conclusion The new elastic distractor is effective in inducing new bone formation.BMP-2 and OPG com-bination acts synergistically,and leads to significant enhancement of bone formation and remodeling. |
| Keywords: | distraction osteogenesis bone morphogenetic protein-2 osteoprotegerin |
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