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| 非小细胞肺癌患者外周血Th17细胞比例和血清IL-17水平的检测及其临床意义 | |
| 引用本文： | 赵树强,贾春生,王桂琴,李淑勤.非小细胞肺癌患者外周血Th17细胞比例和血清IL-17水平的检测及其临床意义[J].肿瘤研究与临床,2012,24(9):600-602. |
| 作者姓名： | 赵树强 贾春生 王桂琴 李淑勤 |
| 作者单位： | 1. 032200,山西医科大学附属汾阳医院核医学科 2. 山西医科大学微生物学与免疫学教研室 3. 山西医科大学汾阳学院临床系 |
| 基金项目： | 山西省吕梁市科技攻关项目（st2010143-1） |
| 摘 要： | 目的 探讨非小细胞肺癌(NSCLC)患者外周血Th17细胞比例和自细胞介素-17(IL-17)水平的变化及其与患者临床病理的关系.方法 收集60例原发未治疗的NSCLC患者(NSCLC组)和20例健康志愿者(对照组),采用流式细胞术(FCM)检测Th17细胞比例,采用酶联免疫吸附试验(ELISA)检测血清IL-17水平.结果 NSCLC组Th17细胞占CD+3 T细胞比例高于对照组(1.23±0.41)％比(1.05±0.28)％,t=1.679,P=0.097],鳞状细胞癌患者外周血Th17细胞比例明显高于腺癌患者(1.31±0.39)％比(1.09±0.41)％,t=2.093,P=0.041].NSCLC组IL-17水平明显高于健康对照组(21.11±7.87) pg/ml比(17.64±5.07)pg/ml,t=2.280,P=0.027],鳞状细胞癌患者血清IL-17表达水平明显高于腺癌患者(23.11±8.73) pg/ml比(18.54±6.38)pg/ml,t=2.203,P=0.032].Th17细胞比例、IL-17的表达水平随临床分期的进展呈下降趋势F=3.151,P=0.032;F=4.132,P=0.010].结论 NSCLC患者外周血中Th17细胞比例和IL-17的表达与肿瘤病理分型、TNM分期相关,可能在NSCLC的病程中发挥重要作用. |
| 关 键 词： | 癌 非小细胞肺 流式细胞术 Th17细胞 白细胞介素-17 |
Detection and clinical significance of Thl7 cell proportion and expression of IL-17 in peripheral bloodof patients with non-small cell lung cancer |
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| ZHAO Shu-qiang , JIA Chun-sheng , WANG Gui-qin , LI Shu-qin.Detection and clinical significance of Thl7 cell proportion and expression of IL-17 in peripheral bloodof patients with non-small cell lung cancer[J].Cancer Research and Clinic,2012,24(9):600-602. | |
| Authors: | ZHAO Shu-qiang JIA Chun-sheng WANG Gui-qin LI Shu-qin |
| Affiliation: | . Department of Nuclear Medicine, Affiliated Fenyang Hospital of Shanxi Medical University, Fenyang 032200, China |
| Abstract: | Objective To investigate the relationship between the proportion of Thl7 cells and IL-17 expression in the peripheral blood and their clinical significance in patients with non-small cell lung cancer （NSCLC）. Methods The proportion of Th 17 cells determined by flow cytometry （FCM） and IL-17 detected by enzyme-linked immunosorbent assay （ELISA）. The data was analyzed from 60 untreated patients with NSCLC （NSCLC group） and 20 healthy volunteers （control group）. Results The Thl7 cells proportion in CD; T-cells in NSCLC group was significantly higher than that in control group （1.23±0.41） % vs （1.05±0.28） %, t = 1.679, P = 0.097]. The expression levels of Thl7 cells in peripheral blood of patients with squamous cell carcinoma was significantly higher than that in adenoeareinoma patients （1.31±0.39） % vs （1.09±0.41） %, t = 2.093, P = 0.041]. The concentration of IL-17 of patients with NSCLC was significantly higher than that in control group （21.11±7.87） pg/m] vs （17.64±5.07） pg/ml, t = 2.280, P = 0.027]. The expression levels of serum IL-17 of squamous cell carcinoma patients was significantly higher than that of adenocarcinoma patients （23.11± 8.73） pg/ml vs （18.54±6.38） pg/ml, t = 2.203, P = 0.032]. As clinical stage progressed, the proportion of Thl7 cells and IL-17 expression were significantly downtrend IF = 3.151, P = 0.032, F = 4.132, P = 0.010]. Conclusion The levels of Thl7 cells and 1L-17 in the peripheral blood of patients with NSCLC significantly correlated with the pathological types and TNM stage, might play an important role in the course of the disease. |
| Keywords: | Carcinoma non-small cell lung Flow cytometry Th17 cells Interleukin-17 |
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