Immunohistochemical characterization of rat thymic non-lymphoid cells. II. Macrophages and granulocytes defined by monoclonal antibodies. |
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| Authors: | M Coli&#x , L J Popovi&#x , S Gasi&#x , V Dragojevi&#x -Simi&#x , N M Mili&#x evi&#x , D Matanovi&#x , A Duji&#x |
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| Affiliation: | Institute for Experimental Medicine, Military Medical Academy, Belgrade, Yugoslavia. |
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| Abstract: | A panel of monoclonal antibodies (mAb) raised to antigens of rat thymic non-lymphoid cells (predominantly macrophages and granulocytes) was immunohistochemically characterized. Based on their staining patterns on cryostat thymic sections and double labellings using acid phosphatase activity, anti-cytokeratin mAb to exclude binding to epithelium or ED1 and ED2 mAb, specific for rat macrophages, antibodies were subdivided into four groups: (i) R-MC 39 mAb strongly reactive with macrophages in the cortex and cortico-medullary zone (CMZ) and weakly with some scattered macrophages in the medulla, blood vessels and thymocytes; (ii) R-MC 40, 41 and 42 mAb specific for cortical macrophages and most CMZ macrophages; (iii) R-MC 43 and 44 mAb predominantly recognizing CMZ and medullary macrophages; (iv) R-MC 45 mAb strongly labelling granulocytes and weakly a subset of macrophages throughout the thymus and isolated cells in the medulla. The obtained results show considerable heterogeneity within mobile thymic non-lymphoid cells and the presence of specific or common antigens in macrophages of particular topographic localization in the rat thymus. |
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