| DKK1对乳腺癌细胞迁移侵袭能力影响的研究 |
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| 引用本文： | 王学涵,王福刚,田刚.DKK1对乳腺癌细胞迁移侵袭能力影响的研究[J].中国肿瘤临床,2013,40(20):1221-1223. |
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| 作者姓名： | 王学涵 王福刚 田刚 |
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| 作者单位： | ①.新疆医科大学第五附属医院检验科（乌鲁木齐市830011） |
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| 基金项目： | 本文课题受天津市滨海新区卫生局科研基金项目(编号2100BHKL004，2100BHKY023)资助@@@@This work was supported by the Scientific Research Funds of Tianjin Binhai Prefectural Municipal Bureau of Health |
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| 摘 要： | 目的 探讨Dikkopf 1（DKK1）对乳腺癌细胞迁移侵袭能力的影响。 方法 采用免疫组织化学方法检测DKK1在乳腺癌组织中的表达，并通过免疫荧光对DKK1在细胞中的定位进行分析；DKK1过表达的真核表达载体转染乳腺癌细胞MDA231，应用Western blot检测转染后乳腺癌细胞MDA231中DKK1蛋白表达水平。划痕实验检测转染后MDA231细胞的迁移能力，Transwell实验检测MDA231细胞侵袭能力的改变。 结果 免疫组织化学检测到乳腺癌组织原发病灶及转移病灶DKK1表达明显高于对应的癌旁组织（P < 0.05）。DKK1表达的阳性率在有淋巴结转移组织中明显比无淋巴结转移组织中低。免疫荧光结果显示DKK1主要呈细颗粒状散在分布于细胞质中。Western blot检测表明DKK1过表达明显。过表达DKK1的MDA231细胞迁移和侵袭能力明显比阴性对照组和空白组低。 结论 DKK1的表达与乳腺癌细胞迁移和侵袭能力呈负相关。
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| 关 键 词： | 乳腺癌 DKK1 迁移 侵袭 |
| 收稿时间： | 2013-07-15 |
Effects of DKK1 on breast cancer cell migration and invasion capa-bility |
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| Xuehan WANG
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Fugang WANG
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Gang TIAN.Effects of DKK1 on breast cancer cell migration and invasion capa-bility[J].Chinese Journal of Clinical Oncology,2013,40(20):1221-1223. |
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| Authors: | Xuehan WANG Fugang WANG Gang TIAN |
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| Affiliation: | ①.Department of Clinical Laboratory, Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China②.Department of Clinical Laboratory, People's Hospital of Kelamayi, Kelamayi 834000, China③.Department of Clinical Laboratory, Tianjin Gong'an Hospital, Tianjin 300040, China |
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| Abstract: | Objective To analyze the DKK1 expression in breast cancer cell line MDA-MB-231 and examine its effect on the migration and invasion of the cell line. Method DKK1 expression in the breast cancer tissues was detected immunohistochemically, and DKK1 distribution in the breast cancer cells was observed using immunofluorescence. Western blot was used to investigate DKK1 expression in breast cancer tissues and their matched normal breast tissues. A eukaryotic expression vector of DKK1 was constructed and transfected into MDA231 cell line. The migration and invasion ability of the cells were observed through scratch assay and Boyden chamber, respectively. Results Immunohistochemical staining showed that DKK1 in the transfected lymph node or non-lymph node was highly expressed in the breast cancer tissues (P < 0.05). The positive DKK1 expression in lymph node metastasis was less significant than those in non-lymph node transfected cells. Immunofluorescence localization results showed a remarkable bright red granular fluorescence in the cytoplasm of the MDA231 cell line. Western blot analysis showed that the DKK1 protein expression in carcinoma tissues were obviously higher than that in matched normal breast tissues. The migration and invasion ability of DKK1- overexpressed MDA231 cell was lower than those of MDA231 cell transfected with pCMV-Tag-2b empty vector. Conclusion Negative correlation was found between DKK1 expression and the migration and invasion ability in breast cancer cells. |
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| Keywords: | breast cancer DKK1 migration invasion |
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