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| 应用细胞片层技术构建功能性组织工程骨的动物实验研究 | |
| 引用本文： | Chen T,Wang Y,Bu L,Li N. 应用细胞片层技术构建功能性组织工程骨的动物实验研究[J]. 华西口腔医学杂志, 2011, 29(4): 442-445. DOI: 10.3969/j.issn.1000-1182.2011.04.027 |
| 作者姓名： | Chen T Wang Y Bu L Li N |
| 作者单位： | 青岛大学医学院附属医院口腔颌面外科, 青岛266003 |
| 基金项目： | 国家自然科学基金资助项目(30872896); 山东省自然科学基金资助项目(Y2008C77) |
| 摘 要： | 目的 细胞片层技术与传统的骨组织工程方法相结合构建功能性组织工程骨.方法 密度梯度离心法分离培养犬骨髓基质干细胞(BMSCs);将向成骨细胞诱导后的BMSCs接种至温度反应性培养皿中,37 ℃、5%CO<,2>饱和湿度培养,然后降温至20℃制备BMSCs细胞片层;制备犬脱钙骨基质(DBM)及富血小板血浆(PRP);将D... |
| 关 键 词： | 骨髓基质干细胞 细胞片层技术 组织工程骨 |
| 收稿时间： | 2011-08-25 |
| 修稿时间： | 2011-08-25 |
An animal experiment of construction of functional tissue-engineered bone with cell sheet technology |
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| Chen Tao,Wang Yanhui,Bu Lingxue,Li Ningyi. An animal experiment of construction of functional tissue-engineered bone with cell sheet technology[J]. West China journal of stomatology, 2011, 29(4): 442-445. DOI: 10.3969/j.issn.1000-1182.2011.04.027 | |
| Authors: | Chen Tao Wang Yanhui Bu Lingxue Li Ningyi |
| Affiliation: | Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China |
| Abstract: | Objective To construct functional tissue-engineered bone with cell sheet technology and method of traditional bone tissue engineering. Methods Canine bone marrow mesenchymal stem cells（BMSCs）were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 ℃, 5%CO2 and saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 ℃. Demineralized bone matrix（DBM） and platelet-rich plasma（PRP） were prepared, and complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. Complex of DBM/PRP/BMSCs was implanted under the right latissimus dorsi muscle. Results When temperature dropped at 20 ℃, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/ BMSCs group. Conclusion Cell sheet technology combined with traditional bone tissue provides a new way for construction of ideal functional tissue-engineered bone. |
| Keywords: | bone marrow mesenchymal stem cells cell sheet technology tissue-engineered bone |
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