管福来,尤敏,孙延龙,李文通,王金冬,吴晓燕,姜子童,李娇,刘志军.海藻提取物对病毒诱导的1型糖尿病中Vβ13基因表达和Treg细胞的影响（英文）[J].中国海洋药物,2017,36(3):34-70.
海藻提取物对病毒诱导的1型糖尿病中Vβ13基因表达和Treg细胞的影响（英文）
Effects of seaweed extract on Vβ13 gene expression and Treg cells in virus-induced type 1 diabetes mellitus
投稿时间：2016-10-21  修订日期：2016-10-28
DOI：10.13400/j.cnki.cjmd.2017.03.006
中文关键词:  巨细胞病毒  1型糖尿病  海藻提取物  Vβ13基因  Treg细胞
English Keywords:cytomegalovirus  type 1 diabetes  seaweed extract  Vβ13  Treg cells
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管福来,尤敏,孙延龙,李文通,王金冬,吴晓燕,姜子童,李娇,刘志军 潍坊医学院 
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中文摘要:
      目的：测定巨细胞病毒（KRV）感染大鼠脾淋巴细胞中Vβ13mRNA和Treg细胞的表达，以及海藻提取物对1型糖尿病的治疗效果。方法40只大鼠随机分为两组。使用与KRV组合的大鼠巨细胞病毒建立1型糖尿病大鼠模型。治疗组中的大鼠用海藻提取物每天治疗40天，而实验组给予相同量的无菌盐水。通过RT-PCR评估脾脏中Vβ13mRNA的表达，并通过流式细胞术（FCM）检测CD4 CD25 Treg细胞。通过HE染色检测脾细胞中Vβ13mRNA的表达。结果：在实验组中，90％的大鼠患有糖尿病（18/20），平均血糖水平为3.15g / L。实验组Vβ13的表达高于治疗组。实验组中CD25 Treg细胞的比例为6.2％，治疗组为13.5％（P <0.05）。结论病毒性糖尿病早期Vβ13mRNA的表达显着高于实验组，CD4 CD25 Treg细胞比例较低。海藻提取物增加Treg细胞表达可以用于治疗糖尿病。
English Summary:
      Objective To measure the expression of Vβ13 mRNA and Treg cells in spleen lymphocytes from rats infected with cytomegalovirus (KRV) and the therapeutic effects of seaweed extract on type 1 diabetes mellitus. Methods Forty rats were randomly divided into two groups. The rat model of type 1 diabetes mellitus was established using rat cytomegalovirus combined with KRV. The rats in the treatment group were treated with seaweed extract daily for 40 days, while the experimental group was given the same amount of sterile saline. The expression of Vβ13 mRNA in the spleen was assessed by RT-PCR and CD4 CD25 Treg cells were detected by flow cytometry (FCM). The expression of Vβ13 mRNA in splenic cells was detected by HE staining. Results In the experimental group, 90% of the rats were diabetic (18/20), and the mean blood glucose level was 3.15 g/L. The expression of Vβ13 in the experimental group was higher than that in the treatment group. The ratio of CD25 Treg cells in the experimental group was 6.2% and in the treatment group 13.5% (P <0.05). Conclusion The expression of Vβ13 mRNA was significantly higher in the early stage of virus-induced diabetes, and the proportion of CD4 CD25 Treg cells was lower in the experimental group. Seaweed extract increased the Treg cell expression for the treatment of diabetes.
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