The mechanism of circRNA_000809 in regulating endometriosis stromal cell proliferation, migration and epithelial-mesenchymal transition by targeting miR-200c-3p
1.Department of Obstetrics and Gynecology, the Second Affliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 2.Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
XU Chaoyi,CHEN Haiyan,CHEN Sailing, et al. The mechanism of circRNA_000809 in regulating endometriosis stromal cell proliferation, migration and epithelial-mesenchymal transition by targeting miR-200c-3p[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(7): 532-538.
Abstract：Objective: To explore the role of circRNA_000809 in regulating proliferation, migration and epithelial-mesenchymal transition (EMT) of endometriosis stromal cells by targeting miR-200-3p. Methods: From January 2020 to January 2022, twenty-five cases of chocolate ovarian cyst tissues from patients undergoing ovarian cyst excision in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University were selected as the endometriosis group. Twenty-five normal endometrial tissue samples were collected from non-endometriosis patients of childbearing age who underwent hysterectomy or hysteroscopy for early cervical cancer and were assigned to the normal endometrial group. qRT-PCR analysis was used to detect the expression of circRNA_000809 and miR-200c-3p in ectopic endometrium tissues of endometriosis patients and normal endometrium. The 5-ethynyl-2’-deoxyuridine (EdU) assays were used to detect cell proliferation ability. Transwell migration assays were employed to monitor migration capabilities. Western blot was performed to detect protein expression. Results: The circRNA_000809 expression in ectopic endometrium tissues was increased (P<0.05), and the miR-200c-3p expression was decreased compared with normal endometrium tissues (P<0.05). Pearson’s test showed that the expression of circRNA_000809 and miR-200c-3p was negatively correlated in ectopic endometrium tissues. We co-transfected ectopic endometrial stromal cells with siNC+inhibitor NC, si-circRNA_000809+inhibitor NC and si-circRNA_000809+miR-200c-3p inhibitor, respectively. The results showed that the proliferation and migration of ectopic endometrial stromal cells were attenuated and the protein levels of ZEB1/ZEB2 were also decreased in the si-circRNA_000809+inhibitor NC group compared with the siNC+inhibitor NC group; while the proliferation and migration of ectopic endometrial stromal cells and the expression of ZEB1/ZEB2 protein were reversed to some extent in the si-circRNA_000809+miR-200c-3p inhibitor group (P<0.05). Conclusion: Silencing of circRNA_000809 inhibited EESCs proliferation, migration, and EMT through miR-200c-3p/EMT signaling.
