兔骨髓间充质干细胞向成骨方向的诱导分化及体内应用

doi:吉林省科技厅资助课题

兔骨髓间充质干细胞向成骨方向的诱导分化及体内应用

韩冬^1△，李建军²，张巨¹，孙鸿斌¹，王健³，刘建国²，王国君^1*

1.　吉林大学中日联谊医院手外科，吉林　长春130031；2.吉林大学第一医院骨科，吉林长春130021；3. 温州医学院附属第一医院骨科，浙江温州 325000

收稿日期:2003-05-21 修回日期:1900-01-01 出版日期:2004-01-28 发布日期:2004-01-28
通讯作者: 王国君

Experimental study of osteoblastic differentiationof rabbit messenchymal stem cell in vitro and in vivo

HAN Dong^1△, LI Jian-jun², ZHANG Ｊu¹, SUN Hong-bin¹, WANG Jian³, LIU Jian-guo²,WANG Guo-jun^1*

1. Deptartment of Hand Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031, China;2. Department of Orthopaedics, First Hospital, Jilin University, Changchun 130031, China;3. Department of Orthopaedics, First Hospital, Wenzhou Medical College，Wenzhou 325000, China

Received:2003-05-21 Revised:1900-01-01 Online:2004-01-28 Published:2004-01-28
Contact: WANG Guo-jun

摘要/Abstract

摘要： 目的：研究经矿化诱导的兔骨髓间充质干细胞(MSC)在裸鼠体内的成骨潜能。方法：分离并培养兔MSC，矿化诱导，观察MSC的形态学改变及碱性磷酸酶（ALP）活性。将诱导后的MSC-去抗原牛松质骨复合体植入裸鼠背部皮下，3 d后取材，行组织学观察。结果:经矿化诱导后，MSC的形态由长梭形向多角形和三角形转化，ALP组织化学染色阳性，活性明显增高。MSC-去抗原牛松质骨复合体植入裸鼠后3 d，组织学见细胞均匀贴附于骨小梁表面，伸展良好，未见未贴壁细胞及外源性纤维组织、血管长入。结论：经矿化诱导的MSC向成骨细胞方向分化和增殖，经观察MSC-去抗原牛松质骨复合体在裸鼠体内可成活并增殖。

关键词: 间充质干细胞, 成骨细胞, 去抗原异种松质骨, 疾病模型, 动物

Abstract: Objective\ To study the osteogenic property of the induced rabbit bone messenchymal stem cells(MSC) in nude rats. Methods MSC were obtained by using gradient centrifuge method. The cells had been cultured in mineralized medium for 1 week, then the ALP activity was observed. The induced MSC/antigen-extracted bovine cancellous bone were implanted in the nude mice subcutaneously. At 3rd day after implantation, the implants underwent the histological analysis. Results ALP activity was higher and the cells displayed fibroblast-like morphology, revealed positive staining with alkaline phosphatase method in the induced medium. At 3rd day after implantation, histological analysis revealed that the induced cells were adhered at the surface of the bone trabeculase and growed well. Exogenous fibro-tissue, blood vessel and free induced cells were invisible. Conclusion Rabbit MSC could differentiate into osteoblast and proliferate induced by the mineral medium. The induced MSC/antigen-extracted bovine cancellous bone could survive in the nude rat.

Key words: messenchymal stem cell, osteoblasts, antigen-extracted xenogeneic cancellous bone, disease models, animal

中图分类号:

Q254

韩冬，李建军，张巨，孙鸿斌，王健，刘建国，王国君. 兔骨髓间充质干细胞向成骨方向的诱导分化及体内应用[J]. J4, 2004, 30(1): 79-81.

HAN Dong, LI Jian-jun, ZHANG Ｊu, SUN Hong-bin, WANG Jian, LIU Jian-guo,WANG Guo-jun. Experimental study of osteoblastic differentiationof rabbit messenchymal stem cell in vitro and in vivo[J]. J4, 2004, 30(1): 79-81.

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