Objective:To explore the risk factors of childhood acute lymphoblastic leukemia(ALL) in Chinese. Methods:A case-control study including 233 cases and 380 controls and univariate and multivariate logistic regression were employed to evaluate the association of the factors in childhood ALL. Analysis was carried out with SPSS13.0 software. Results:In the univariate logistic analysis,subjects living without parents,with immature labor,un-spontaneous delivery,biocide exposure,paternal alcohol exposure,actino-exposure,houses painting and chemical exposure were the statistically significant risk factors of childhood ALL. Multivariate logistic analysis also revealed that living without parents,with immature labor,biocide exposure,paternal alcohol exposure,and houses painting were the risk factors. Conclusion:Living without parents,with immature labor,biocide exposure,paternal alcohol exposure,and houses painting are associated with the childhood ALL.