| 姚燕箐,汪焱,徐文静,等.异落新妇苷通过调节NLRP3炎症小体和NF-κB信号通路减轻尿酸钠诱导的小鼠急性痛风性关节炎机制研究[J].浙江中医药大学学报,2022,46(9):929-935, 944. |
| 异落新妇苷通过调节NLRP3炎症小体和NF-κB信号通路减轻尿酸钠诱导的小鼠急性痛风性关节炎机制研究 |
| Mechanism Study of Isoastilbin on Attenuating MSU-Induced Acute Gouty Arthritis in Mice Through Modulating NLRP3 Inflammasome and NF-κB Signaling Pathway |
| DOI：10.16466/j.issn1005-5509.2022.09.001 |
| 中文关键词: 异落新妇苷 急性痛风性关节炎 尿酸钠 踝关节肿胀 炎症因子 NLRP3炎症小体 NF-κB信号通路 机制研究 |
| 英文关键词: isoastilbin acute gouty arthritis MSU ankle swelling inflammatory factor NLRP3 inflammasome NF-κB signaling pathway mechanism research |
| 基金项目:国家自然科学基金项目(82074085 81673656);浙江省自然科学址金项(211H000;浙江中医药大学省重点建设高校优势特色学科(中药学)开放基金重点项目(ZYAOXZD2019002) |
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| 中文摘要: |
| [目的] 基于NOD样受体蛋白3(NOD-like receptor pyrin domain 3，NLRP3)炎症小体和核因子-κB(nuclear factor-κB，NF-κB)信号通路，研究异落新妇苷(isoastilbin，IA)对小鼠急性痛风性关节炎模型的抗炎作用及机制。[方法] 通过关节腔注射尿酸钠(monosodium urate，MSU)混悬液，建立小鼠急性痛风性关节炎模型，以自制足趾容积测量装置测定踝关节肿胀度；苏木精-伊红(hematoxylin-eosin，HE)染色法观察踝关节炎性浸润情况；酶联免疫吸附试验(enzyme linked immunosorbent assay，ELISA)检测踝关节中白细胞介素-1β(interleukin-1β，IL-1β)、白细胞介素-6(interleukin-6，IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α，TNF-α)的水平；免疫印迹法检测踝关节中NLRP3炎症小体和NF-κB信号通路中相关蛋白的表达情况。 [结果] 踝关节注射MSU混悬液诱导小鼠产生急性痛风性关节炎。与正常组比较，模型组小鼠踝关节肿胀率明显升高(P＜0.01)，炎性浸润增加，IL-1β、IL-6、TNF-α分泌水平显著增高(P＜0.01)，NLRP3炎症小体和NF-κB信号通路相关蛋白表达增加(P＜0.01)。与模型组比较，IA具有较强的抗炎效果，剂量依赖性地降低小鼠踝关节肿胀率(P＜0.05，P＜0.01)，减轻炎性浸润情况，减少相关炎症因子的分泌(P＜0.01)，下调NLRP3炎症小体和NF-κB信号通路相关蛋白表达(P＜0.01)。 [结论] IA对小鼠急性痛风性关节炎模型表现出较强的抗炎效果，其机制与调节NLRP3炎症小体和NF-κB信号通路相关。 |
| 英文摘要: |
| [Objective] To study the anti-inflammatory effect and mechanism of isoastilbin(IA) on acute gouty arthritis in mice based on NOD-like receptor pyrin domain 3(NLRP3) inflammasome and nuclear factor-κB(NF-κB) signaling pathway. [Methods] The acute gouty arthritis model in mice was established by injecting monosodium urate(MSU) suspension into the articular cavity. Ankle swelling rate was measured by self-made toe volume installation; the state of inflammatory infiltration within ankle was observed through hematoxylin-eosin (HE) staining; the level of interleukin-1β (IL-1β)， interleukin-6 (IL-6)， tumor necrosis factor-α (TNF-α) secreted from ankle was determined by enzyme linked immunosorbent assay(ELISA); the expression of protein in ankle linked to NLRP3 inflammasome and NF-κB signaling pathway was detected by Western blot. [Results] MSU suspension was injected into ankle induced acute gouty arthritis in mice. Compared with normal group， the swelling rate of ankle in model group was significantly increased(P＜0.01)， inflammatory infiltration was increased， the secretion level of IL-1β， IL-6 and TNF-α was significantly increased(P＜0.01)， and the expression of NLRP3 inflammasome and NF-κB signaling pathway related proteins were increased(P＜0.01). Compared with model group， IA had a good anti-inflammatory effect. IA reduced the swelling rate of ankle in a dose-dependent manner(P＜0.05， P＜0.01)， alleviated inflammatory infiltration， reduced the secretion of related inflammatory cytokines(P＜0.01)， and down-regulated the expression of NLRP3 inflammasome and NF-κB signaling pathway related proteins(P＜0.01). [Conclusion] IA has a strong anti-inflammatory effect in mice on acute gouty arthritis， and its mechanism is related to the regulation of NLRP3 inflammasome and NF-κB signaling pathway. |
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