Objective: To observe the effect of ursolic acid on insulin resistance and possible mechanisms. Methods: The insulin resistance model were made by high fat diet. Detect the fasting blood glucose（FBG）, fasting serum insulin（FIN）, the oral glucose tolerance test（OGTT）, the content of the liver glucokinase, the expression of mRNA and protein of glucokinase.Calculate insulin sensitivity index (ISI) and insulin resistance index (HOMA-IR).Results: In ursolic acid 300mg/（kg·d）group and ursolic acid 150mg/（kg·d）group, the results of FINS, HOMA-IR were decreased, ISI was incresed, and OGTT was improved. The levels of glucokinase content, mRNA and protein expression in the liver were elevated obviously.Conclusion：It is suggested that the therapeutic effects of ursolic acid on insulin resistance might be related to the glucokinase expression in the liver of insulin resistance rats.