Objective: The research that the juvenile male juvenil rats serum testosterone and testicular tissue (cyto-chrome P450 side chain cleavage,P450scc) was impacted by Tripterygium wilfordii glycosides (GTW) and its intervention role Bushen Chinese herbs (flavonoids from Cuscuta chinensis, Liuwei Dihuang Wan). Method: SD 3-4 week-old 48 male rats were randomly divided into 4 groups: control group(CMC-Na), GTW(9 mg·kg^-1·d^-1),Liuwei Dihuang Wan Group (Liuwei Dihuang Wan 1 g·kg^-1·d^-1＋GTW 9 mg·kg^-1·d^-1),flavonoids from C.chinensis group(flavonoids from C.chinensis 0.1 g·kg^-1·d^-1＋GTW 9 mg·kg^-1·d^-1).After 12 weeks, rats were sacrificed,the blood serum was collected from the rats for the determination of serum testosterone;specimens of testicular tissue for the detection of P450scc in the expression of the gene and protein level by two experimental methods using RT-PCR and Western blot. Result: GTW can significantly decrease serum testosterone values,GTW group and blank group comparison there is a significant difference (P<0.01), Liuwei group with GTW group can significantly increase serum testosterone values,Liuwei group and GTW group comparison GTW group have significant significant difference (P<0.05);GTW at the protein level can significantly decrease the testicular tissue P450scc value,GTW group P450scc protein expression values were lower than other groups, GTW group and blank group comparison have significant difference (P<0.05); GTW cannot reduce the testicular tissue P450scc value at the genetic level,GTW group and other groups showed no significant difference. Conclusion: Prompted GTW certain inhibitory effect on the reproductive system of male juvenil rats; prompted Liuwei Dihuang Wan can significantly improve male rats serum testosterone levels and achieve the role of protecting reproductive damage.