Objective: To investigate the therapeutic mechanism of Yinchen Wulingsan based on network pharmacology. Method: Based on the Traditional Chinese Medicine Systems Pharmacology Database, the Protein Databank and the String database, the chemical compositions of Yinchen Wulingsan, corresponding targets and diseases were obtained respectively. Meanwhile, compound-target network, as well as target-disease network and protein-protein interaction network, were constructed. According to biological information annotation dotabase(DAVID) annotation databases, gene ontology biology process (GO-BP) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the targets were performed. Result: Totally 58 components, 103 targets, 11 hub targets and 262 diseases were obtained. The key targets involved tumor necrosis factor (TNF), peroxidase synthetase 2 (PTGS2), and estrogen receptor (ESR1), etc. The diseases mainly involved tumor, immune and endocrine. There were 146 GO-BP entries, including signal transduction entries, transcription and translation entries, proliferation promotion and apoptosis inhibition entries, cellular component entries, and oxidative stress entries. Besides, there were 108 KEGG pathways, involving tumor, virus, parasite and so on. Conclusion: The targets and mechanism corresponding to compounds of Yinchen Wulingsan were studied systematically in this study, providing ideas for further clinical researches and new drug development.