Objective: To assay the inhibitive effect on seven kinds of human tumor cells of rhamnocitrin and rhamnazin flavonoids compounds obtained from Nervilia fordii(Hance) Schltr.Methods: Anticancer experiment in vitro was done by applying MTT method to iveatigation of rhamnocitrin and rhamnazin on human leukemia L1210 and P388D1,uterine carcinoma Hela,gastric adenocarcinoma SGC7901,inhibiting cell B16,neuromas NG108-15,Human hepatoma Hele7404.Results: Rhamnocitrin inhibited the growth of L1210,P388D1,Hela,B16,NG108-15 and Hele7404 human tumor cells.With the concentration elevated,inhibiting action increased.But rhamnocitrin had no effects on SGC7901,and rhamnazin had no effects on seven kinds of human tumor cells.Conclusion: Rhamnocitrin,one of the active principles of Nervilia fordii(Hance) Schltr,inhibited the growth of human tumor cells in vitro.However,rhamnazin had no effects.