目的研究黄蘑Hohenbuehelia serotina多糖对环磷酰胺(CTX)化疗H22荷瘤小鼠的减毒和增效作用。方法建立小鼠体内移植性肝癌模型;小、中、大剂量治疗组分别ip黄蘑多糖提取物20、40、80mg/kg,连续给药10d,测定荷瘤小鼠的生命延长率和化疗药物CTX的毒副作用以及黄蘑多糖对CTX的减毒和增效作用。同时检测黄蘑多糖对淋巴细胞转化率、IL-2水平等免疫系统功能的影响。结果中、大剂量黄蘑多糖具有抑制肿瘤生长,延长荷瘤小鼠的生存率的作用。与CTX伍用可发挥协同作用,提高抑瘤率(P<0.05、0.01);提高荷瘤小鼠生存质量,体重、白细胞计数及免疫器官指数,与CTX阳性对照组比较均显示显著差异(P<0.05、0.01);实验结果显示黄蘑多糖与CTX伍用具有良好的减毒和增效作用,可增强荷瘤小鼠机体免疫力,提高淋转和IL-2的水平(P<0.05、0.01)。结论黄蘑多糖作为生物反应调节剂可提高机体的免疫力,增强CTX的抗肿瘤作用,同时减轻CTX的毒性。

Objective To study the toxicity-decreasing and synergistic effect of Hohenbuehelia serotina polysaccharides on H₂₂ mice treated by cytoxan (CTX). Methods The tumor-bearing mice models with Hepatoma-22 were established and divided into three groups. H. serotina polysaccharides extracts were administered with dosages of 20, 40, and 80 mg/(kg·d) through ip for 10 d, respectively to observe their life prolongable rates, the side and toxic reactions of CTX, lymphocyte transformations, and IL-2 levels. Results The group treated with large and medium dosages showed H. serotina polysaccharides could inhibit the tumor development with increasing the survival rate, have good synergism and attenuation of toxicity with CTX, enhance the inhibition on growth of Hepatoma (P<0.05, 0.01), increase the weight, WBC count, and immune system index of tumor-bearing mice (P<0.05, 0.01), stimulate the immune system, and improve lymphocyte transformation and IL-2 levels (P<0.05, 0.01). Conclusion As biological response modifier, H. serotina polysaccharides can increase the activity of body immunity, improve the anti-tumor effects of CTX, and attenuate the toxicity of CTX as well. They should be studied and exploited furtherly.

吉林省科技发展计划项目资助课题(20020803)