目的研究灯盏花种质资源间的遗传关系。方法对采自云南、贵州和四川等地的36份灯盏花种质资源进行ISSR遗传多样性分析和NTSYS2聚类分析。结果筛选出的13个引物在36份种质资源中共产生125条DNA片段, 其中103条带具有遗传多态性, 约占82.40%。当遗传相似性系数为0.54时, 可以把灯盏花分为5大类, 第Ⅰ大类包括采自楚雄、昭通、迪庆等地的7份种质资源;第Ⅱ大类可分为2个亚类, ⅡA包括采自滇中及滇西北的5份种质资源, ⅡB包括采自滇东北、贵州、四川的20份种质资源;第Ⅲ大类包括采自文山的1份种质资源;第Ⅳ大类包括采自曲靖的1份种质资源;第Ⅴ大类包括采自迪庆的2份种质资源。结论灯盏花种质资源遗传变异丰富, 遗传关系与其采集地的地理分布距离具有一定的相关性, 但并非严格按地理界线聚在一起。

Objective To investigate the genetic relationships of Erigeron breviscapus at the molecular biology level. Methods Thirty six germplasm resources of E.breviscapus collected from Yunnan,Sichuan,and Guizhou Provinces in 2005 were analyzed by inter-simple sequence repeat(ISSR) and cluster analysis based on NTSYS2.Results A total of 13 primers were screened and 125 bands were amplified,among which 103 bands(82.40%) were found to be polymorphic.Thirty six germplasm resources of E.breviscapus were clustered into five groups at genetic distance 0.54,the group Ⅰ included five germplasm resources collected from Chuxiong,Zhaotong,and Diqing of Yunnan Province; the group Ⅱ included 25 germplasm resources collected from Guizhou,Sichuan,and Northwest of Yunnan Province;group Ⅲ included one germplasm resource collected from Wenshan of Yunnan Province;the group Ⅳ included one germplasm resource collected from Qujing of Yunnan Province;the group Ⅴ included two germplasm resources collected from Diqing of Yunnan Province.Conclusion There is abundant genetic diversity in the germplasm resources of E.breviscapus,and the genetic relationships are closely related to geographical distance where they are collected.

国家“十五”攻关项目(2004BA721A34);云南省“十一五”社会发展攻关项目(2006SG016);国家自然科学基金(30760301,30660040)