目的建立当归属药用植物功效的分子系统学基础，为当归属药用植物功效的差异性提供分子依据。方法采用PCR技术与克隆技术相结合，获得ITS基因，进行测序，经Phylip软件统计分析和分支分析，建立系统发育树，计算各类群间的遗传距离。结果当归属药用植物中ITS1-5.8 S-ITS2序列长度为600~629 bp，去除序列长度为165 bp的5.8S，ITS1+ITS2排序后的长度为468个位点。系统树将当归属药用植物分为两组，以独活、白芷为代表分别聚类，甘肃产岷当归Angelica sinensis不与上述任何一组聚类，却与欧当归Levisticum officinale遗传距离相对较小。结论上述结果与当归属药用植物功效差异基本吻合，启示药用植物亲缘演化关系可能是研究药用植物功效规律的一条重要途径。

Objective To establish the base of molecular system-theory on medicinal plants of Angelica L. and to offer molecular gists for finding the efficacy difference of them. Methods The PCR technique was utilized together with the clone technique to get the ITS gene and measure its genie sequence. Statistical analyzing and branch analyzing were carried out by Phylip software and genetic distance difference in every kinds can be calculated with MEGA3. Results ITSl一5. 8 S-ITS2, belongs to one of medicinal plants of Angelica L., its sequence length extents 600 to 629 bp. Subtracting 5. 8 S, its length is 165 by, the length of ITS is 468 bp. The medicinal plants of Angelica L. are divided into two groups by dendrogram. The genetic distance of A. sinensis from Gansu Province is obviously different from the other medicinal plants of Angelica L., but the distance difference compared with Levisicum officinale is smaller. Conclusion It suggests that the above result be identical to the efficacy of medicinal plants of Angelica L. It may be an important reason of explaining why the efficacy between A. sinensis and other medicinal plants of Angelica L. is so different.

国家自然科学基金项目资助(30271618)